Mag-Bind Viral DNA/RNA 96 Kit
Viral Nucleic Acid Isolation Kit
Mag-Bind® Viral DNA/RNA Kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from serum, swabs, plasma, saliva, and other body fluids. The Mag-Bind® magnetic beads technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down depending on the amount of starting sample. The purified nucleic acids are ready for direct use in downstream applications such as amplification or other enzymatic reactions.Protocols are available for the following automated platforms:
Coronavirus (SARS-CoV-2) Extraction
Omega Bio-tek is assisting scientists, researchers and healthcare workers around the globe in accelerating the screening and detection of the novel coronavirus disease, COVID-19. We are supporting several Laboratory Developed Tests (LDTs) by providing high-throughput, automated viral nucleic acid extraction methodologies. To meet the exceptional and immediate need for supplies, Omega Bio-tek has ramped up the production of its Mag-Bind® Viral DNA/RNA 96 kit and has the capacity to support 1 million patient tests per month.
- Fully automatable and ready-to-load scripts for:KingFisher™: Email firstname.lastname@example.org for scriptHamilton Microlab STAR: 384 samples in ~3 hrHamilton MagEx STARlet: 96 samples in ~1 hr 45 min
- Dedicated technical and application support to expedite setup and validation time.
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||50 µL - 200 µL|
|Starting Material||Serum, plasma, saliva and other body fluids|
|Elution Volume||20-50 μL|
|Processing Mode||Automated, Manual|
|Throughput||8 - 96|
Protocol and Resources
Product Documentation & Literature
Mag-Bind ® Viral DNA/RNA Kit had better yield than a leading competing product
Figure 1. HBV virus (in quantities of 10 and 1 infectious unit[s]) was spiked into 200 µL human serum. Viral nucleic acid was isolated with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and with a comparable kit from Company A according to recommended protocols. 5 µL of template was used for a SYBR® Green-labeled qPCR reaction which was replicated 4 times. The resulting mean Ct values are shown in the above figure.
DNA/RNA purified with Mag-Bind® Viral DNA/RNA had less inhibitor than with a leading competing product
Figure 2. Nucleic acid was isolated from 200 µL of human whole blood with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and a comparable kit from Company A using the manufacturer’s recommended protocols. The extractions were eluted in 100 µL. Three concentrations of template were used as templates in a SYBR® Green-labeled qPCR reaction. Each reaction was performed in quadruplicate and the mean Ct value is depicted in the above figure.
Figure 3. 50 µL of ZeptoMetrix’s NATtrol Influenza A/B Positive Control standard was spiked into 150 µL of human serum and viral nucleic acids were extracted using Omega Bio-tek’s Mag-Bind® Viral DNA/RNA 96 Kit. Average Ct values obtained after amplification using Influenza B primers are shown above. The results indicate positive detection of Influenza B in both undiluted and 10-fold diluted purified samples.
Table 1. Some of the viruses* detected using our viral kits.
|Influenza A||Hepatitis E||Sheep pox virus|
|Influenza B||Infectious Bronchitis virus||Murine norovirus 1|
|West Nile virus||Porcine reproductive and respiratory syndrome Virus (PRRSV)||Canine distemper virus|
|Middle East Respiratory Syndrome Coronavirus (MERS-CoV)||Insect-specific flaviviruses, mononegaviruses, and totiviruses||Rabies virus|
|Zika virus (ZIKAV)||orf virus (ORFV)||Rotavirus|
|SIV||Porcine circovirus type 2 (PCV2)||Coxsackievirus B3|
|Influenza A (H1N1)||Dengue virus||Avian leukosis virus subgroup J|
|Hepatitis A virus types 1 and 3||GB virus C||Avian Encephalomyelitis Virus|
|Hepatitis B virus||Bovine Viral Diarrhea Virus (BVDV)||Crimean-Congo hemorrhagic fever virus|
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- Yu, Yongzhong, et al. “Characterization of an Orf Virus Isolate from an Outbreak in Heilongjiang Province, China.” Archives of Virology, vol. 162, no. 10, June 2017, pp. 3143–49, doi:10.1007/s00705-017-3426-x.
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- Bliss, N., et al. “Prevalence of Influenza A Virus in Exhibition Swine during Arrival at Agricultural Fairs.” Zoonoses and Public Health, vol. 63, no. 6, Jan. 2016, pp. 477–85, doi:10.1111/zph.12252.
- McCorkell, Robert, et al. “Acute BVDV-2 Infection in Beef Calves Delays Humoral Responses to a Non-Infectious Antigen Challenge.” The Canadian Veterinary Journal, vol. 56, no. 10, Oct. 2015, pp. 1075–1083, www.ncbi.nlm.nih.gov/pmc/articles/PMC4572827/.
- Solis Worsfold, Cristina, et al. “Assessment of Neutralizing and Non-Neutralizing Antibody Responses against Porcine Circovirus 2 in Vaccinated and Non-Vaccinated Farmed Pigs.” Journal of General Virology, vol. 96, no. 9, Sept. 2015, pp. 2743–48, doi:10.1099/vir.0.000206.
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- Yan, Mengfei, et al. “Infection of Porcine Circovirus 2 (PCV2) in Intestinal Porcine Epithelial Cell Line (IPEC-J2) and Interaction between PCV2 and IPEC-J2 Microfilaments.” Virology Journal, vol. 11, no. 1, Nov. 2014, doi:10.1186/s12985-014-0193-0.