Mag-Bind® Total RNA 96 Kit | Total RNA Purification
The Mag-Bind® Total RNA 96 Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA and viral RNA from a wide variety of cells and tissues. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves the removal of contaminants and increases nucleic acid purity. Mag-Bind® Total RNA 96 Kit procedure can be fully automated with most robotic workstations.
Protocols are available for the following automated platforms:
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, Next Generation Sequencing, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||30-50 µL|
|Starting Material||Animal Tissue and Cultured Cells|
|Starting Amount||1 x 106 cells or 10 mg tissue|
|Processing Mode||Automated; Manual|
|Throughput||Up to 96|
|RNA Binding Technology||Magnetic Beads|
Protocol and Resources
Product Documentation & Literature
RNA purified using Mag-Bind® Total RNA 96 Kit was of high quality
Figure 1. RNA Integrity. Total RNA was extracted from 1×106 cells using the Mag-Bind® Total RNA Kit. The RNA samples were analyzed with RNA tape on Agilent’s TapeStation® 2200.
Yield Comparison vs Leading Competitor
Figure 2. RNA Yield Comparison. RNA was isolated using the Mag-Bind® Total RNA Kit and a comparable column-based kit from Company Q. Elution volumes for company Q were 50 µL; for Omega were 100 µL, RNA was quantified using Thermo Scientific’s NanoDrop® 2000c
RNA Yield From Mouse Liver
Figure 3. 10 mg mouse liver was isolated with the Mag-Bind Total RNA Kit according to protocol. Total RNA concentration determined by optical density measurements using Thermo Scientific’s NanoDrop® 2000c. Total elution volume was 100 µL
Frequently Asked Questions
Yes, you can as long as you follow some protocol modifications. At step 5 on page 6, add 400 µL of 100% ethanol instead of 300 µL. At step 26 on page 8, add 480 µL of RNA Wash Buffer II instead of 300 µL.
Homogenized cell/tissue lysates in OTRK Lysis Buffer can be stored at -80 °C for at least 6 months. To proceed with the frozen lysates, thaw the sample at 37 °C until they are completely thawed, and all salts in the lysis buffer are dissolved. Do not extend the treatment in 37 °C because it can cause chemical degradation of RNA.
The RNAlater will interfere with our buffer chemistry resulting in low yields so tissues must be washed before starting the extraction.
- Spin down the tissue at 500 x g for 5 minutes.
- Aspirate out the supernatant.
Resuspend the pellet with 1% PBS Buffer.
- Spin down the sample again (500 x g for 5 minutes), then aspirate out the supernatant.
- Use the pelleted tissue for extraction following the Mag-Bind Total RNA 96 Kit Protocol- Tissue on page 5.
- Qichao Huang, Biaoyang Lin, Hanqiang Liu, Xi Ma, Fan Mo, Wei Yu, Lisha Li, Hongwei Li, Tian Tian, Dong Wu, Feng Shen, Jinliang Xing, Zhi-Nan Chen. RNA-seq analyses generate comprehensive transcriptomic landscape and reveal complex transcript patterns in hepatocellular carcinoma