Mag-Bind® Blood & Tissue DNA HDQ 96 Kit
Genomic DNA Isolation From Blood and Tissue
The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is designed for isolation of high-quality genomic DNA from blood samples, saliva, swabs, mouse tails, dried blood spots or cultured cells. Mag-Bind® Particles HDQ provides quick magnetic response time reducing overall processing time. This system combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with Omega Bio-tek’s tissue and blood DNA isolation systems. Utilizing paramagnetic particles provides high-quality DNA that is suitable for direct use in most downstream applications such as Next Generation Sequencing, qPCR, PCR, and microarrays. The Mag-Bind Blood & Tissue DNA HDQ 96 Kit can be scaled up to process 1 mL whole blood samples.
For larger volumes, the Mag-Bind® Blood DNA HV Kit can be used.
- Safe – No organic solvents
- Compatible with DNA Genotek’s preservation system
- Compatible with Mawi iSWAB DNA preservation system
- High quality – Purified DNA suitable for downstream applications such as Next Generation Sequencing, qPCR, PCR, and microarrays
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 106 cultured cells.|
|Elution Volume||50-200 µL|
|Processing Mode||Automated, Manual|
|Throughput||8 - 384|
|Note||Downstream applications: NGS, qPCR, microarray|
Protocol and Resources
Product Documentation & Literature
DNA yield from 200 μL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument.
Figure 1. DNA yield from 200 µL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument. DNA yield was determined by PicoGreen® quantification.
DNA yield from buccal swab samples using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is higher than a leading competitor.
Figure 2. Genomic DNA was extracted from buccal swab samples using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit and Company A’s genomic DNA extraction kit. 10% of DNA extracted from each sample was analyzed on a 0.8% agarose gel.
Purified genomic DNA using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is of high quality and free of inhibitors.
Figure 3. Genomic DNA was extracted from 1,500 µL whole blood using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit. The elution volume was 500 µL. DNA was analyzed on Thermo Scientific’s NanoDrop® 2000c. 50 ng of DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.
DNA purified using Mag-Bind Blood & Tissue DNA HDQ 96 Kit was around 60 kb
Figure 4. The sizes of genomic DNA purified using Mag-Bind Blood & Tissue DNA HDQ Kit were determined by electrophoresis. Sample 1 and 2 were gDNA from human blood. L is 50 kb genomic ladder for Agilent TapeStation 2200. The size of gDNA is around 60 kb.
Frequently Asked Questions
Yes, they can be made into a master mix before starting the experiment.
- Cut or punch-out the blood spot from the filter paper (up to 200 µL blood can be used per spot). Tear or cut the filter paper into small pieces and place them into a 2-mL nuclease-free microcentrifuge tube.
- Add 250 µL PBS.
- Incubate at 65 °C for 1 hour. Vortex briefly every 20 minutes.
- Add 25 µL Proteinase K Solution. Vortex at maximum speed for 15 seconds.
- Incubate at 65 °C for 30 minutes. Vortex briefly several times during incubation.
- Centrifuge at ≥13,000 x g for 5 minutes.
- Transfer the supernatant to a nuclease-free 2-mL microcentrifuge tube.
- Add 290 µL AL Buffer and vortex, or pipet up and down 20 times, to mix. Optional: Add 5 µL RNase A to each sample. Vortex to mix. Let sit at room temperature for 2 minutes.
- Continue from step 5 (page 6) of the ‘Blood Protocol’ (250 µL) on the Mag-Bind Blood & Tissue DNA HDQ 96 Kit manual.
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