Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit
DNA Extraction From Blood & Tissue
The Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit includes prefilled plates in 96-well standard automation format. The configuration of this kit works seamlessly on magnetic processors such as the Thermo Fisher Scientific KingFisher® Flex, QIAGEN BioSprint®, and Applied Biosystems MagMAX® Express-96. Included in this kit are all plastic accessories, including tip combs, that are needed for an extraction run post-lysis. This kit offers a versatile method for isolating high-quality DNA from a wide variety of samples, including fresh or frozen animal cultured cells and tissues, buccal swabs, mouse tail snips, dried blood spots, up to 250 μL whole blood, and up to 250 μL saliva.With no reagent set-up and the quick magnetic response of the Mag-Bind® Particles PF-HDQ, hands-on time and the overall processing time are significantly reduced. The purification procedure provides high-quality DNA suitable for direct use in most downstream applications including amplification, next generation sequencing (NGS), and enzymatic reactions.
Features and Benefits
- Ease of Use – Reduction of hands-on time with no reagent preparation (for bind, wash, and elute steps)
- Convenience – Inclusion of tip combs and other required plasticware in the kit for user convenience
- Extraction Accuracy – Reduction of user-associated errors
- Safe – No organic solvents
- High-Quality – Purified DNA suitable for downstream applications such as next-generation sequencing, qPCR, PCR, and microarrays
For Research Use Only. Not for use in diagnostic procedures.
|Starting Material||Whole blood, saliva, swabs, mouse tails, buffy coat, dried blood spots, cultured cells|
|Starting Amount||Variable (depending on the sample input)|
|Elution Volume||100 µL|
|Processing Mode||Automated, Post-lysis|
|Throughput||4 x 96|
|Nucleic Acid Binding Technology||Magnetic Beads|
|Run Time||~40 minutes
For scripting support, contact one of our automation support specialists.
|Downstream Application(s)||qPCR, NGS, microarray, etc|
|Storage Conditions|| Store all components at room temperature in an upright position.
Proteinase K Solution at 2-8°C
Protocol and Resources
Product Documentation & Literature
Average DNA Yield from Tissue and Whole Blood Samples
Table 1. The average DNA yield from 250 μL whole blood samples and 10 mg mouse kidney tissue samples were obtained using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit and eluted in 100 μL volume. DNA yield was determined using Thermo Scientific’s NanoDrop® 2000c. The absorbance ratios > 1.8 indicate high purity of the isolated DNA.
TapeStation® Analysis on Purified DNA from Mouse Kidney Tissue Samples
Figure 2. The size of genomic DNA purified from mouse kidney tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit was analyzed on the Agilent TapeStation® 2200. The purified DNA is of high molecular weight, with the software analyzing it to be > 60 kb. The DIN values are all > 8.2, indicating highly intact DNA of superior quality.
Average Ct Values from Diluted DNA
Figure 3. Average Ct values obtained amplifying the purified DNA from blood and tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit . The ΔCt value was ~3.3, indicating high-quality DNA that is free of inhibitors.