Mag-Bind® Viral RNA Xpress Kit
$3,950.00
Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads. (24×96 preps)
Overview
Mag-Bind® Viral RNA Xpress Kit follows a magnetic bead-based approach for the rapid and reliable isolation of viral RNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM). The extraction methodology is easily adaptable to various automated systems and can also be scaled up or down depending on the amount of starting sample amount used. The kit utilizes the proven Mag-Bind® technology that enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. The purified nucleic acids are ready for direct use in downstream applications such as qPCR, RT-qPCR and more.
Benefits over Mag-Bind® Viral DNA/RNA 96 Kit
- Improved downstream performance
- Faster protocol
- No Proteinase K step
- Smaller packaging with double the number of preps for reduced shipping costs
- 10% savings on cost per prep
Protocols are available for the following automated platforms:
- Hamilton Microlab® STAR
- Hamilton Microlab® MagEx STARlet
- Hamilton Microlab® NIMBUS
- KingFisher™, BioSprint®, and MagMAX® 96
- Tecan DreamPrep™ NAP
- Adaptable to other liquid handling platforms
Coronavirus (SARS-CoV-2) Extraction
Omega Bio-tek is assisting scientists, researchers, and healthcare workers around the globe in accelerating the screening and detection of the novel coronavirus disease, COVID-19. We are supporting several Laboratory Developed Tests (LDTs) by providing high-throughput, automated viral nucleic acid extraction methodologies. To meet the exceptional and immediate need for supplies, Omega Bio-tek created a more robust viral RNA extraction kit, Mag-Bind® Viral RNA Xpress Kit, and has the capacity to support 6 million patient tests per month.
- Optimal formulation to extract viral RNA from NP swabs, aspirates and BAL samples
- Fully automatable and ready-to-load scripts for:
KingFisher™: Email product_support@omegabiotek.com for script
Hamilton Microlab STAR: (4×96) 384 samples in ~3 hr
Hamilton MagEx STARlet: 96 samples in ~1 hr 45 min - Dedicated technical and application support to expedite setup and validation time.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Starting Amount | 50 µL - 200 µL |
| Starting Material | NP swabs in UTM/VTM as well as dry |
| Elution Volume | 50-100 µL |
| Technology | Magnetic Beads |
| Processing Mode | Automated, Manual |
| Throughput | 8 - 96 |
| Preps | 2304 |
Kit Components
| Item | Available Separately |
|---|---|
| Mag-Bind® Particles RQ | Call for Pricing |
| TNA Lysis Buffer | View Product |
| Carrier RNA | --- |
| RMP Wash Buffer | --- |
| Nuclease-free Water | View Product |
Protocol and Resources
Product Documentation & Literature
SALES SHEET
Product Data
Detection of Synthetic SARS-CoV-2 virus control following RT-qPCR
Figure 1. 1×105 copies of synthetic SARSCoV-2 was spiked into a 200 μL sample containing 2000 HEK293 cells. Viral nucleic acids were extracted following the recommended protocol from Mag-Bind® Viral RNA Xpress Kit (M6219). 4 and 8 μL of template was used in a 20 μL SYBR Greenlabeled RT-qPCR reaction. The average Ct values obtained are shown on the left. The Ct difference between the two template 30.89 amounts is ~1 indicating no qPCR inhibition.
Detection of Influenza B virus following RT-qPCR: Mag-Bind® Viral RNA Xpress Kit vs Mag-Bind® Viral DNA/RNA 96 Kit
Figure 2. 50 µL of Influenza B virus control was spiked into a 200 μL sample containing 2000 HEK293 cells. Viral nucleic acids were extracted following the recommended protocols from Mag- Bind® Viral RNA Xpress Kit (M6219) and Mag-Bind® Viral DNA/RNA 96 Kit (M6246). 4 and 8 μL of template was used in a 20 μL SYBR Green-labeled RT-qPCR reaction. The average Ct values obtained are shown left. The Ct difference between the two template amounts used is ~1 indicating no qPCR inhibition. The Ct values for the Mag-Bind® Viral RNA Xpress Kit (M6219) were on an average ~0.5 Ct lower than Mag-Bind® Viral DNA/RNA 96 Kit (M6246) indicating improved performance.
Citations
- Bliss, N., et al. “Prevalence of Influenza A Virus in Exhibition Swine during Arrival at Agricultural Fairs.” Zoonoses and Public Health, vol. 63, no. 6, Jan. 2016, pp. 477–85, doi:10.1111/zph.12252.
- Chotiwan, Nunya, et al. “Rapid and Specific Detection of Asian- and African-Lineage Zika Viruses.” Science Translational Medicine, vol. 9, no. 388, May 2017, doi:10.1126/scitranslmed.aag0538.
- Dow, Natalie, et al. “Genetic Variability of Bovine Viral Diarrhea Virus and Evidence for a Possible Genetic Bottleneck during Vertical Transmission in Persistently Infected Cattle.” PLOS ONE, edited by Binu T Velayudhan, vol. 10, no. 7, July 2015, p. e0131972, doi:10.1371/journal.pone.0131972.
- Eschbaumer, Michael, et al. “Probe-Free Real-Time Reverse Transcription Polymerase Chain Reaction Assays for the Detection and Typing of Porcine Reproductive and Respiratory Syndrome Virus in Canada.” Canadian Journal of Veterinary Research, vol. 79, no. 3, July 2015, pp. 170–179, www.ncbi.nlm.nih.gov/pmc/articles/PMC4445508/.
- Fauver, Joseph R., Shamima Akter, et al. “A Reverse-Transcription/RNase H Based Protocol for Depletion of Mosquito Ribosomal RNA Facilitates Viral Intrahost Evolution Analysis, Transcriptomics and Pathogen Discovery.” Virology, vol. 528, Feb. 2019, pp. 181–197, doi:10.1016/j.virol.2018.12.020.
- Fauver, Joseph R., Brian D. Foy, et al. “The Use of Xenosurveillance to Detect Human Bacteria, Parasites, and Viruses in Mosquito Bloodmeals.” The American Journal of Tropical Medicine and Hygiene, vol. 97, no. 2, Aug. 2017, pp. 324–29, doi:10.4269/ajtmh.17-0063.
- Fauver, Joseph R., James Weger-Lucarelli, et al. “Xenosurveillance Reflects Traditional Sampling Techniques for the Identification of Human Pathogens: A Comparative Study in West Africa.” PLOS Neglected Tropical Diseases, edited by Paulo Filemon Pimenta, vol. 12, no. 3, Mar. 2018, p. e0006348, doi:10.1371/journal.pntd.0006348.
- Gloria-Soria, A., et al. “Infection Rate of Aedes Aegyptimosquitoes with Dengue Virus Depends on the Interaction between Temperature and Mosquito Genotype.” Proceedings of the Royal Society B: Biological Sciences, vol. 284, no. 1864, Oct. 2017, p. 20171506, doi:10.1098/rspb.2017.1506.
- Grubaugh, Nathan D., et al. “Transmission Bottlenecks and RNAi Collectively Influence Tick-Borne Flavivirus Evolution.” Virus Evolution, vol. 2, no. 2, July 2016, doi:10.1093/ve/vew033.
- Gu, Shao-peng, et al. “Identification and Phylogenetic Analysis of the Sheep Pox Virus Shanxi Isolate.” Www.Biomedres.Info, 2018, www.biomedres.info/biomedical-research/identification-and-phylogenetic-analysis-of-the-sheep-pox-virus-shanxi-isolate-9658.html.
- Law, Jessica, et al. “Induction of Humoral Immune Response in Piglets after Perinatal or Post-Weaning Immunization against Porcine Circovirus Type-2 or Keyhole Limpet Hemocyanin.” Canadian Journal of Veterinary Research, vol. 81, no. 1, Jan. 2017, pp. 5–11, www.ncbi.nlm.nih.gov/pmc/articles/PMC5220598/.
- McCorkell, Robert, et al. “Acute BVDV-2 Infection in Beef Calves Delays Humoral Responses to a Non-Infectious Antigen Challenge.” The Canadian Veterinary Journal, vol. 56, no. 10, Oct. 2015, pp. 1075–1083, www.ncbi.nlm.nih.gov/pmc/articles/PMC4572827/.
- Prakash, Ravi, et al. “Detection of Arboviruses in Blood and Mosquito Slurry Samples Using Polymer Microchip.” IEEE Xplore, 1 Nov. 2017, pp. 168–171, doi:10.1109/HIC.2017.8227611.
- Puryear, Wendy Blay, et al. “Prevalence of Influenza A Virus in Live-Captured North Atlantic Gray Seals: A Possible Wild Reservoir.” Emerging Microbes & Infections, vol. 5, no. 1, Jan. 2016, pp. 1–9, doi:10.1038/emi.2016.77.
- Rückert, Claudia, et al. “Impact of Simultaneous Exposure to Arboviruses on Infection and Transmission by Aedes Aegypti Mosquitoes.” Nature Communications, vol. 8, May 2017, doi:10.1038/ncomms15412.
- Solis Worsfold, Cristina, et al. “Assessment of Neutralizing and Non-Neutralizing Antibody Responses against Porcine Circovirus 2 in Vaccinated and Non-Vaccinated Farmed Pigs.” Journal of General Virology, vol. 96, no. 9, Sept. 2015, pp. 2743–48, doi:10.1099/vir.0.000206.
- Spivey, Timothy J., et al. “Maintenance of Influenza A Viruses and Antibody Response in Mallards (Anas Platyrhynchos) Sampled during the Non-Breeding Season in Alaska.” PLOS ONE, edited by Balaji Manicassamy, vol. 12, no. 8, Aug. 2017, p. e0183505, doi:10.1371/journal.pone.0183505.
- Yan, Mengfei, et al. “Infection of Porcine Circovirus 2 (PCV2) in Intestinal Porcine Epithelial Cell Line (IPEC-J2) and Interaction between PCV2 and IPEC-J2 Microfilaments.” Virology Journal, vol. 11, no. 1, Nov. 2014, doi:10.1186/s12985-014-0193-0.
- Yu, Yongzhong, et al. “Characterization of an Orf Virus Isolate from an Outbreak in Heilongjiang Province, China.” Archives of Virology, vol. 162, no. 10, June 2017, pp. 3143–49, doi:10.1007/s00705-017-3426-x.
| Format | Magnetic beads |
|---|
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