Mag-Bind® Viral RNA Xpress Kit follows a magnetic bead-based approach for the rapid and reliable isolation of viral RNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM). The extraction methodology is easily adaptable to various automated systems and can also be scaled up or down depending on the amount of starting sample amount used. The kit utilizes the proven Mag-Bind® technology that enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. The purified nucleic acids are ready for direct use in downstream applications such as qPCR, RT-qPCR and more.
Benefits over Mag-Bind® Viral DNA/RNA 96 Kit
- Improved downstream performance
- Faster protocol
- No Proteinase K step
- Smaller packaging with double the number of preps for reduced shipping costs
- 10% savings on cost per prep
Protocols are available for the following automated platforms:
Coronavirus (SARS-CoV-2) Extraction
Omega Bio-tek is assisting scientists, researchers, and healthcare workers around the globe in accelerating the screening and detection of the novel coronavirus disease, COVID-19. We are supporting several Laboratory Developed Tests (LDTs) by providing high-throughput, automated viral nucleic acid extraction methodologies. To meet the exceptional and immediate need for supplies, Omega Bio-tek created a more robust viral RNA extraction kit, Mag-Bind® Viral RNA Xpress Kit, and has the capacity to support 6 million patient tests per month.
- Optimal formulation to extract viral RNA from NP swabs, aspirates and BAL samples
- Fully automatable and ready-to-load scripts for:
KingFisher™: Email email@example.com for script
Hamilton Microlab STAR: (4×96) 384 samples in ~3 hr
Hamilton MagEx STARlet: 96 samples in ~1 hr 45 min
- Dedicated technical and application support to expedite setup and validation time.
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||50 µL - 200 µL|
|Starting Material||NP swabs in UTM/VTM as well as dry|
|Elution Volume||50-100 µL|
|Processing Mode||Automated, Manual|
|Throughput||8 - 96|
Protocol and Resources
Product Documentation & Literature
Detection of Synthetic SARS-CoV-2 virus control following RT-qPCR
Figure 1. 1×105 copies of synthetic SARSCoV-2 was spiked into a 200 μL sample containing 2000 HEK293 cells. Viral nucleic acids were extracted following the recommended protocol from Mag-Bind® Viral RNA Xpress Kit (M6219). 4 and 8 μL of template was used in a 20 μL SYBR Greenlabeled RT-qPCR reaction. The average Ct values obtained are shown on the left. The Ct difference between the two template 30.89 amounts is ~1 indicating no qPCR inhibition.
Detection of Influenza B virus following RT-qPCR: Mag-Bind® Viral RNA Xpress Kit vs Mag-Bind® Viral DNA/RNA 96 Kit
Figure 2. 50 µL of Influenza B virus control was spiked into a 200 μL sample containing 2000 HEK293 cells. Viral nucleic acids were extracted following the recommended protocols from Mag- Bind® Viral RNA Xpress Kit (M6219) and Mag-Bind® Viral DNA/RNA 96 Kit (M6246). 4 and 8 μL of template was used in a 20 μL SYBR Green-labeled RT-qPCR reaction. The average Ct values obtained are shown left. The Ct difference between the two template amounts used is ~1 indicating no qPCR inhibition. The Ct values for the Mag-Bind® Viral RNA Xpress Kit (M6219) were on an average ~0.5 Ct lower than Mag-Bind® Viral DNA/RNA 96 Kit (M6246) indicating improved performance.
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