The Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter’s Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.
- No protocol change against major competitor
- Read lengths averaging over 800 bps (Min Phred 20)
- Manual or adjustable to automated liquid handlers
- Significant cost savings compared to sephadex-based clean ups
- 96- or 384-well formats
For Research Use Only. Not for use in diagnostic procedures.
|Downstream application||Sanger Sequencing, Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting|
|Elution volume||40 µL or above|
|Starting material||ABI Big Dye Chemistry Cycle Sequencing Reactions|
|Starting amount||5 - 20 µL|
|DNA recovery||Sequencing reads start at 40 nt|
|Processing mode||Automated; Manual|
|Throughput||96-384 samples per run|
|DNA binding technology||Magnetic beads|
|Storage||2°C - 8°C|
Protocol and Resources
Product Documentation & Literature
DNA samples treated with Mag-Bind® SeqDTR had superior signal strength and continuous read length than using leading competitors
Figure 1. Purified 1.8 kb PCR fragments were sequenced from each company using the manufacturer’s recommended protocols. The median of 16 samples per company are used in the representations above. A 5 µL sequencing reaction was performed using a 1/32 dilution of Applied Biosystems BigDye Terminator v3.1 chemistry. DNA was analyzed on an Applied Biosystems 3730XL.
Cost Comparison When Using Mag-Bind SeqDTR for Sequencing Clean-up
Table 1. Less BigDye is needed when using Mag-Bind SeqDTR for sample prep and clean up. Therefore reduce cost in BigDye usage. Mag-Bind SeqDTR cost per prep is significantly lower than the competitor.
- Dang, Meng, et al. “Identification, Development, and Application of 12 Polymorphic EST-SSR Markers for an Endemic Chinese Walnut (Juglans Cathayensis L.) Using next-Generation Sequencing Technology.” Biochemical Systematics and Ecology, vol. 60, 1 June 2015, pp. 74–80, www.sciencedirect.com/science/article/pii/S0305197815000800?casa_token=zjCcQoePc6MAAAAA:ACf_Ktz3-WuYKigbix3T-lT0uePcinMMpt_pFFtZJwmZlgCWG8sYWDTnLukoH5ZYt3sPBuuP6nk, 10.1016/j.bse.2015.04.004. Accessed 1 June 2020.
- Huang, Wei, et al. “Formation and Determination of the Oxidation Products of 5-Methylcytosine in RNA.” Chemical Science, vol. 7, no. 8, 2016, pp. 5495–5502, pubs.rsc.org/–/content/articlehtml/2016/sc/c6sc01589a, 10.1039/C6SC01589A. Accessed 1 June 2020.
- Jiang, Han-Peng, et al. “Determination of Formylated DNA and RNA by Chemical Labeling Combined with Mass Spectrometry Analysis.” Analytica Chimica Acta, vol. 981, 15 Aug. 2017, pp. 1–10, www.sciencedirect.com/science/article/pii/S0003267017307122?casa_token=xt2DarDWL_QAAAAA:VNeMkTQYF8rqKrXCSIozXzlMQPcKL7OifWYX3AXJvn7yDqT_jGdgwyuNbs1IEJTr5l0d2iWobNs, 10.1016/j.aca.2017.06.009. Accessed 1 June 2020.
- Kim, Chun Hwan, et al. “Genomic DNA Data of FT-IR Spectroscopy Could Discriminate from Different Species of Artichoke (Cynara Cardunculus Var. Scolymus L).” 한국원예학회 학술발표요지, 1 Oct. 2015, www.dbpia.co.kr/Journal/articleDetail?nodeId=NODE06561860. Accessed 1 June 2020.
- Li, Wei, et al. “Multilocus Genotypes and Broad Host-Range of Enterocytozoon Bieneusi in Captive Wildlife at Zoological Gardens in China.” Parasites & Vectors, vol. 9, no. 1, 8 July 2016, 10.1186/s13071-016-1668-1. Accessed 1 June 2020.
- Li, Xiaoqi, et al. “Response of Soil Microbial Communities and Microbial Interactions to Long-Term Heavy Metal Contamination.” Environmental Pollution, vol. 231, 1 Dec. 2017, pp. 908–917, www.sciencedirect.com/science/article/pii/S0269749117309636?casa_token=pX1gd-8Pvy8AAAAA:PCKA2h8pnm7-JEDgTaFt3CNbs7eJKhiza8mbjGaqSe8Fzrt0AYShPH5jKC5igcP-6rjOr2h6-UE, 10.1016/j.envpol.2017.08.057. Accessed 1 June 2020.
- Li, Yan, et al. “Host Range, Prevalence, and Genetic Diversity of Adenoviruses in Bats.” Journal of Virology, vol. 84, no. 8, 15 Apr. 2010, pp. 3889–3897, jvi.asm.org/content/84/8/3889.short, 10.1128/JVI.02497-09. Accessed 1 June 2020.
- Mao, Yaqing, et al. “Avian Leukosis Virus Contamination in Live Vaccines: A Retrospective Investigation in China.” Veterinary Microbiology, 11 May 2020, p. 108712, www.sciencedirect.com/science/article/pii/S0378113520303011?casa_token=_wGLiK3RXZQAAAAA:nUBr9H_S0ReEc-uNIf7EC0lMCJcQzrqblmWXEfCLzUp9oEDfj5qu6dkImYlw5wFa3oTtlKBOQIw, 10.1016/j.vetmic.2020.108712. Accessed 1 June 2020.
- Schuster, Andre, et al. “A Versatile Toolkit for High Throughput Functional Genomics with Trichoderma Reesei.” Biotechnology for Biofuels, vol. 5, no. 1, 2012, p. 1, 10.1186/1754-6834-5-1. Accessed 1 June 2020.
- Song, Seung Yeob, et al. “Establishment of Discrimination System Using Multivariate Analysis of FT-IR Spectroscopy Data from Artichoke (Cynara Scolymus Var. Scolymus L) Flower Buds.” 한국원예학회 학술발표요지, 1 Oct. 2015, www.dbpia.co.kr/Journal/articleDetail?nodeId=NODE06561861. Accessed 1 June 2020.
- Sun, Qi-Bao, et al. “SSR and AFLP Markers Reveal Low Genetic Diversity in the Biofuel Plant Jatropha Curcas in China.” Crop Science, vol. 48, no. 5, 2008, pp. 1865–1871, dl.sciencesocieties.org/publications/cs/abstracts/48/5/1865, 10.2135/cropsci2008.02.0074. Accessed 1 June 2020.
- Wan, Chun-he, et al. “Complete Genome Sequence of a Novel Duck Parvovirus Isolated in Fujian, China.” Kafkas Universitesi Veteriner Fakultesi Dergisi 22.6 (2016).
- Xu, B. L., et al. “Predominance of the Hungarian Clone (ST 239-III) among Hospital-Acquired Meticillin-Resistant Staphylococcus Aureus Isolates Recovered throughout Mainland China.” Journal of Hospital Infection, vol. 71, no. 3, 1 Mar. 2009, pp. 245–255, www.sciencedirect.com/science/article/pii/S0195670108004507?casa_token=vVwF2f50JlIAAAAA:5PfUrBUVypgqp83cf5Pj0h4DnzTQegYE7AxNKkMxbN-D0whsZv3a197FQ0GxpgFWQfptjsHjUcw, 10.1016/j.jhin.2008.10.029. Accessed 1 June 2020.
- Yang, Y.-W., et al. “Isolation and Characterization of Microsatellite Markers for Amomum Tsaoko (Zingiberaceae), an Economically Important Plant in China.” Genetics and Molecular Research, vol. 13, no. 4, 2014, pp. 8220–8224, geneticsmr.com/sites/default/files/articles/year2014/vol13-4/pdf/gmr3908.pdf, 10.4238/2014.october.8.3. Accessed 1 June 2020.
- Yu, Libo, et al. “Microbacterium Sediminis Sp. Nov., a Psychrotolerant, Thermotolerant, Halotolerant and Alkalitolerant Actinomycete Isolated from Deep-Sea Sediment.” International Journal of Systematic and Evolutionary Microbiology, vol. 63, no. Pt_1, 1 Jan. 2013, pp. 25–30, 10.1099/ijs.0.029652-0. Accessed 1 June 2020.