The Mag-BIND® FFPE RNA 96 kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. The sample is first processed in a special buffer by heating to deactivated RNases/DNase and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then mixed with Mag-BIND® particles to capture the capture RNA. After a few quick wash steps, purified RNA can be eluted with water or low salt buffer.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||30-50 µL|
|Starting Material||FFPE tissue|
|Starting Amount||2-5 paraffin sample sections between 5-10 μm|
|Processing Mode||Automated; Manual|
|Throughput||Up to 96|
|RNA Binding Technology||Magnetic Beads|
Protocol and Resources
Product Documentation & Literature
- MicroRNA-552 enhances metastatic capacity of colorectal cancer cells by targeting a disintegrin and metalloprotease 28
Jian Wang, Hai Li, Yong Wang, Libin Wang, Xiurui Yan, Dong Zhang, Xiaoqiang Ma, Yong Du, Xiaoming Liu, Yinxue Yang