Mag-Bind® FFPE RNA 96 Kit

The Mag-Bind® FFPE RNA 96 Kit is designed for the isolation of RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes a specially formulated buffer system that not only partially reverses the formaldehyde-induced crosslinking but also ensures inhibitor-free purification of DNA. Magnetic bead-based extraction makes it suitable for both manual as well as automated processing on most open-ended liquid handling platforms as well as magnetic processors.

The Mag-Bind® FFPE RNA 96 Kit integrates a unique buffer system with the highly efficient binding properties of Mag-Bind® technology to isolate RNA. The protocol utilizes non-toxic mineral oil in combination with heat for efficient deparaffinization of the FFPE sample eliminating the use of hazardous xylene.

Samples are first lysed in FDR Buffer aided by the presence of Proteinase K enzyme. The lysate is then heated to denature the proteinase and reverse the chemical crosslinking of the nucleic acids. Post-heating, the lysate is mixed with MB4 Buffer, isopropanol and Mag-Bind® Particles CH to bind nucleic acids to the particles. Genomic DNA bound to the beads is eliminated through a DNase I digestion step while preserving the integrity of the RNA bound to the beads. The beads are subjected to several wash steps to remove contaminants. High-quality RNA is then eluted in Endo-free water and is ready for use in a wide range of downstream applications such as RT-PCR, RT-qPCR, microarray analysis, next-generation RNA sequencing (RNA-Seq) etc.