Mag-Bind® FFPE DNA/RNA 96 Kit is designed for the sequential isolation of DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes non-toxic mineral oil in the place of the hazardous xylene for efficient deparaffinization of the FFPE sample. The specially formulated buffers reverse cross-linking without the need for overnight digestion resulting in high-yielding, high-quality nucleic acids. The isolation protocol allows for the extraction of both DNA and RNA in separate eluates from the same sample for a comprehensive analysis of both the nucleic acids. Purified DNA and RNA are suitable for a variety of downstream applications including SNP analysis, sequencing, and genotyping. The Mag-Bind® system is fully automatable on the Hamilton Microlab® STAR™, Tecan Fluent™, Thermo KingFisher® Flex™, and other open-ended workstations.
- Xylene-free protocol
- Extraction of both DNA and RNA in separate eluates from the same FFPE sample
- No splitting of samples
- Magnetic bead-based purification
- Automation friendly
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Applications||NGS, PCR, qPCR, real-time RT-PCR, microarray, microRNA analysis|
|Elution Volume||50-200 µL|
|Starting Material||FFPE tissue|
|Starting Amount||< 3 FFPE sections of 10 μm thickness each|
|Throughput||Up to 96|
|Processing Mode||Automated, Manual|
|FDR Buffer||Call For Pricing|
|Proteinase K Solution||View Product|
|MB4 Buffer||Call For Pricing|
|Mag-Bind® Particles CH||Call For Pricing|
|RMP Buffer||Call For Pricing|
|Elution Buffer||View Product|
|DNase Digestion Buffer||Call For Pricing|
|Mag-Bind® DNase I||Call For Pricing|
|PHM Buffer||Call For Pricing|
|Nuclease-free Water||View Product|
Protocol and Resources
Product Documentation & Literature
Frequently Asked Questions
Figure 1. Genomic DNA (A) and RNA (B) was sequentially isolated from the same 1 × 10 µm section of the FFPE tumor tissue sample (n=3) using Omega Bio-tek’s Mag-Bind® FFPE DNA/RNA 96 Kit and other manufacturer’s recommended protocols. Purified DNA and RNA was eluted in 100 µL respectively and quantified using Thermo Scientific’s NanoDrop™ 2000c system.
Figure 2. Purity of DNA (A) and RNA (B) isolated using different manufacturer’s kits was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.
Figure 3. Real-time PCR with human-specific primers was performed in triplicate on 10-fold dilution of DNA (A) and RNA (B) eluates respectively. Average Ct values obtained amplifying the purified DNA and RNA from the same FFPE tumor sample (n=3) following the respective manufacturer’s recommended protocols are shown above.