Mag-Bind® Bacterial DNA 96 Kit

$0.00$1,036.10

Isolate DNA from up to gram positive and negative bacteria

  • Reliable – Reproducible DNA purification from a variety of sample sources
  • High yield – Enzymatic digestion for cell lysis

The Mag-BIND® Bacterial DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0.5 mL gram positive or gram negative bacterial culture can be processed each time. The key to the system is Omega Bio-tek’s proprietary Mag-BIND® particles that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. After bacterial cells are collected from culture or picked from an agar plate, the bacterial cell wall is removed by lysozyme digestion, followed by Proteinase K digestion. Following lysis, binding conditions are adjusted and the sample is mixed with Mag-BIND particles to bind DNA. Three rapid wash steps remove trace salts and protein contaminants, and finally, DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount0.5 mL
Starting MaterialWide variety of bacterial species
Elution Volume200 μL
TechnologyMagnetic beads
Processing ModeAutomated, Manual
Throughput96
NoteCH beads

Figure 1. Genomic DNA purified using the Mag-BIND® Bacterial DNA Kit. Purified genomic DNA from various bacteria was isolated with the Mag-BIND® Bacterial DNA Kit. DNA was extracted in duplicate from 1×106 cells harvested during the log-phase for each of the following samples: sample 1 from S. aureus, sample 2 from E. faecalis, sample 3 from P. aeruginosa. Genomic DNA (5% of total purified DNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA. M: Lambda Hind III DNA Marker.

Product Information

Document NameLanguagesLink
Protocol ManualEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DS BufferGHSEnglishenglishghs
DS BufferGHSSpanishspanishghs
DS BufferREACHEnglishenglishreach
DS BufferREACHDanishdanishreach
DS BufferREACHFinnishfinnishreach
DS BufferREACHFrenchfrenchreach
DS BufferREACHGermangermanreach
DS BufferREACHItalianitalianreach
DS BufferREACHNorwegiannorwegianreach
DS BufferREACHSpanishspanishreach
DS BufferREACHSwedishswedishreach
DS BufferWHMSEnglishenglishwhms
DS BufferWHMSFrenchfrenchwhms
DS BufferREACHDutchdutchreach
DS BufferREACHHungarianhungarianreach
DS BufferREACHPortugueseportuguesereach
DS BufferREACHGreekgreekreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
LysozymeGHSEnglishenglishghs
LysozymeGHSSpanishspanishghs
LysozymeREACHEnglishenglishreach
LysozymeREACHDanishdanishreach
LysozymeREACHFinnishfinnishreach
LysozymeREACHFrenchfrenchreach
LysozymeREACHGermangermanreach
LysozymeREACHItalianitalianreach
LysozymeREACHNorwegiannorwegianreach
LysozymeREACHSpanishspanishreach
LysozymeREACHSwedishswedishreach
LysozymeWHMSEnglishenglishwhms
LysozymeWHMSFrenchfrenchwhms
LysozymeREACHGreekgreekreach
Mag-Bind Particles CHGHSEnglishenglishghs
Mag-Bind Particles CHGHSSpanishspanishghs
Mag-Bind Particles CHREACHEnglishenglishreach
Mag-Bind Particles CHREACHDanishdanishreach
Mag-Bind Particles CHREACHFinnishfinnishreach
Mag-Bind Particles CHREACHFrenchfrenchreach
Mag-Bind Particles CHREACHGermangermanreach
Mag-Bind Particles CHREACHItalianitalianreach
Mag-Bind Particles CHREACHNorwegiannorwegianreach
Mag-Bind Particles CHREACHSpanishspanishreach
Mag-Bind Particles CHREACHSwedishswedishreach
Mag-Bind Particles CHWHMSEnglishenglishwhms
Mag-Bind Particles CHWHMSFrenchfrenchwhms
Mag-Bind Particles CHREACHDutchdutchreach
Mag-Bind Particles CHREACHHungarianhungarianreach
Mag-Bind Particles CHREACHPortugueseportuguesereach
Mag-Bind Particles CHREACHGreekgreekreach
MB1 BufferGHSEnglishenglishghs
MB1 BufferGHSSpanishspanishghs
MB1 BufferREACHEnglishenglishreach
MB1 BufferREACHDanishdanishreach
MB1 BufferREACHFinnishfinnishreach
MB1 BufferREACHFrenchfrenchreach
MB1 BufferREACHGermangermanreach
MB1 BufferREACHItalianitalianreach
MB1 BufferREACHNorwegiannorwegianreach
MB1 BufferREACHSpanishspanishreach
MB1 BufferREACHSwedishswedishreach
MB1 BufferWHMSEnglishenglishwhms
MB1 BufferWHMSFrenchfrenchwhms
MSL BufferGHSEnglishenglishghs
MSL BufferGHSSpanishspanishghs
MSL BufferREACHEnglishenglishreach
MSL BufferREACHDanishdanishreach
MSL BufferREACHFinnishfinnishreach
MSL BufferREACHFrenchfrenchreach
MSL BufferREACHGermangermanreach
MSL BufferREACHItalianitalianreach
MSL BufferREACHNorwegiannorwegianreach
MSL BufferREACHSpanishspanishreach
MSL BufferREACHSwedishswedishreach
MSL BufferWHMSEnglishenglishwhms
MSL BufferWHMSFrenchfrenchwhms
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
Proteinase K SolutionREACHGreekgreekreach
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
SPM BufferGHSEnglishenglishghs
SPM BufferGHSSpanishspanishghs
SPM BufferREACHEnglishenglishreach
SPM BufferREACHDanishdanishreach
SPM BufferREACHFinnishfinnishreach
SPM BufferREACHFrenchfrenchreach
SPM BufferREACHGermangermanreach
SPM BufferREACHItalianitalianreach
SPM BufferREACHNorwegiannorwegianreach
SPM BufferREACHSpanishspanishreach
SPM BufferREACHSwedishswedishreach
SPM BufferWHMSEnglishenglishwhms
SPM BufferWHMSFrenchfrenchwhms
SPM BufferREACHDutchdutchreach
SPM BufferREACHHungarianhungarianreach
SPM BufferREACHPortugueseportuguesereach
SPM BufferREACHGreekgreekreach
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