The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral DNA and viral RNA from cell-free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||150 µL|
|Starting Material||Cell free fluids such as plasma, serum, urine and cell culture supernatant|
|Elution Volume||20-50 μL|
|Technology||HiBind® RNA Mini Column|
|Processing Mode||Manual, Centrifugation/Vacuum|
Protocol and Resources
Product Documentation & Literature
Viral RNA extracted using E.Z.N.A.® Viral RNA Kit was PCR inhibitor free
Figure 1. 25 µL of Zeptomatrix Influenza A/B Positive Control was spiked into 125 µL serum samples and then Viral RNA was extracted using the E.Z.N.A.® Viral RNA Kit . 2 µL of Eluted RNA was used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: RNA sample extracted with kit. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template RNA in free of inhibitors.
- Ana Maria Chamoun, Karuppiah Chockalingam, Michael Bobardt, Rudo Simeon, Jinhong Chang, Philippe Gallay, Zhilei Chen PD 404, 182 is a virocidal small molecule that disrupts Hepatitis C virus and human immunodeficiency virus
- Ville Peltola, Matti Waris, Riikka Österback, Petri Susi, Olli Ruuskanen, Timo Hyypiä Rhinovirus transmission within families with children: incidence of symptomatic and asymptomatic infections
- Natalie Rose Dow Genetic variability of bovine viral diarrhea virus in persistently infected cattle