The E.Z.N.A.® Total RNA Kit I provides a straightforward and rapid method for the isolation of up to 100 µg of total RNA from cultured eukaryotic cells or tissues. Multiple samples of 1 x 106 – 1 x 107 eukaryotic cells, or 5-30 mg tissue, can be processed in parallel. After the homogenization process, samples are applied to the HiBind® RNA Mini Column which binds total RNA. Cellular debris and other contaminants are effectively washed away after a few quick wash steps. Total RNA greater than 200 nt is isolated using this kit.
For Research Use Only. Not for use in diagnostic procedures.
FEATURES | SPECIFICATIONS |
---|---|
Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
Elution Volume | 40-70 μL |
Starting Material | Cultured eukaryotic cells and soft tissues |
Starting Amount | < 1 x 107 cells or 30 mg tissue |
RNA Yield | Up to 100 µg |
Processing Mode | Manual, centrifugation/vacuum |
Throughput | 1 – 24 |
RNA Binding Technology | Silica Mini spin column |
Binding Capacity | 100 µg |
ITEM | AVAILABLE SEPARATELY |
---|---|
HiBind® RNA Mini Columns | — |
2 mL Collection Tubes | View Product |
TRK Lysis Buffer | View Product |
RNA Wash Buffer I | View Product |
RNA Wash Buffer II | View Product |
Nuclease-free Water | — |
Yield from Mouse Tissue
Figure 1. Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.
Yield from HEK-239 Cells
Figure 2. Total RNA was isolated from 2.5×106 HEK-293 cultured cells with Omega Bio-tek’s E.Z.N.A.® Total RNA Kit I with an elution volume of 50 µL. RNA concentrations were quantified with Thermo Scientific’s NanoDrop® 2000c. RNA quality was assessed with the Agilent TapeStation® 2200.
FAQs
Can samples be stored in TRK Lysis Buffer prior to extraction?
Homogenized tissue/cell lysates in TRK Lysis Buffer can be stored at -80 °C for at least 6 months. To proceed with the frozen lysates, thaw the sample at 37 °C until they are completely thawed, and all salts in the lysis buffer are dissolved. Do not extend the treatment in 37 °C because it can cause chemical degradation of RNA.
I have been getting low 260/230 ratios with my samples. Is there a way to improve this?
Low A260/A230 ratio alludes to salt carryover (guanidine salt is not completely washed off during wash steps). Typically increasing the wash steps improves this ratio – so instead of 2 RNA Wash Buffer II wash steps, do 3.
Is there a way to isolate miRNA using the E.Z.N.A. Total RNA Kit I?
To bind RNA <200 nt, they would need to modify the binding step by adding 1 volume of 100% ethanol (instead of 70% ethanol in Step 5, Page 14, and Step 5, Page 19), and rest of the steps remain as-is. This modification helps bind miRNA along with large RNA and will get co-extracted in the same fraction.
Can I use the E.Z.N.A. Total RNA Kit I for tissues or cells stored in RNAlater™?
The RNAlater will interfere with our buffer chemistry resulting in low yields, so tissues must be washed prior to starting the extraction.
- Spin down the tissue at 500 x g for 5 minutes.
- Aspirate out the supernatant.
- Spin down the sample again (500 x g for 5 minutes), then aspirate out the supernatant.
- Use the pelleted cells or tissue for extraction following the E.Z.N.A. Total RNA Kit I Protocol – Culture Cells on page 11 or E.Z.N.A. Total RNA Kit I Protocol – Animal Tissue on page 17.
Product Information
Safety Data Sheets
Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
---|---|---|---|---|---|
Nuclease-free Water | GHS | Spanish | spanish | ghs | |
Nuclease-free Water | REACH | Greek | greek | reach | |
Nuclease-free Water | REACH | Portuguese | portuguese | reach | |
Nuclease-free Water | REACH | Hungarian | hungarian | reach | |
Nuclease-free Water | REACH | Dutch | dutch | reach | |
Nuclease-free Water | WHMS | French | french | whms | |
Nuclease-free Water | WHMS | English | english | whms | |
Nuclease-free Water | REACH | Swedish | swedish | reach | |
Nuclease-free Water | REACH | Spanish | spanish | reach | |
Nuclease-free Water | REACH | Italian | italian | reach | |
Nuclease-free Water | GHS | English | english | ghs | |
Nuclease-free Water | REACH | English | english | reach | |
Nuclease-free Water | REACH | Danish | danish | reach | |
Nuclease-free Water | REACH | French | french | reach | |
Nuclease-free Water | REACH | Norwegian | norwegian | reach | |
Nuclease-free Water | REACH | German | german | reach | |
Nuclease-free Water | REACH | Finnish | finnish | reach | |
RNA Wash Buffer I | GHS | English | english | ghs | |
RNA Wash Buffer I | WHMS | French | french | whms | |
RNA Wash Buffer I | WHMS | English | english | whms | |
RNA Wash Buffer I | REACH | Swedish | swedish | reach | |
RNA Wash Buffer I | REACH | Spanish | spanish | reach | |
RNA Wash Buffer I | REACH | Norwegian | norwegian | reach | |
RNA Wash Buffer I | REACH | German | german | reach | |
RNA Wash Buffer I | GHS | Spanish | spanish | ghs | |
RNA Wash Buffer I | REACH | English | english | reach | |
RNA Wash Buffer I | REACH | Danish | danish | reach | |
RNA Wash Buffer I | REACH | French | french | reach | |
RNA Wash Buffer I | REACH | Italian | italian | reach | |
RNA Wash Buffer I | REACH | Finnish | finnish | reach | |
RNA Wash Buffer II | GHS | Spanish | spanish | ghs | |
RNA Wash Buffer II | WHMS | French | french | whms | |
RNA Wash Buffer II | WHMS | English | english | whms | |
RNA Wash Buffer II | REACH | Swedish | swedish | reach | |
RNA Wash Buffer II | REACH | Spanish | spanish | reach | |
RNA Wash Buffer II | REACH | Norwegian | norwegian | reach | |
RNA Wash Buffer II | REACH | German | german | reach | |
RNA Wash Buffer II | GHS | English | english | ghs | |
RNA Wash Buffer II | REACH | English | english | reach | |
RNA Wash Buffer II | REACH | Danish | danish | reach | |
RNA Wash Buffer II | REACH | Finnish | finnish | reach | |
RNA Wash Buffer II | REACH | French | french | reach | |
RNA Wash Buffer II | REACH | Italian | italian | reach | |
TRK Lysis Buffer | GHS | English | english | ghs | |
TRK Lysis Buffer | GHS | Spanish | spanish | ghs | |
TRK Lysis Buffer | REACH | English | english | reach | |
TRK Lysis Buffer | REACH | Danish | danish | reach | |
TRK Lysis Buffer | REACH | Finnish | finnish | reach | |
TRK Lysis Buffer | REACH | French | french | reach | |
TRK Lysis Buffer | REACH | German | german | reach | |
TRK Lysis Buffer | REACH | Italian | italian | reach | |
TRK Lysis Buffer | REACH | Norwegian | norwegian | reach | |
TRK Lysis Buffer | REACH | Spanish | spanish | reach | |
TRK Lysis Buffer | REACH | Swedish | swedish | reach | |
TRK Lysis Buffer | WHMS | English | english | whms | |
TRK Lysis Buffer | WHMS | French | french | whms |