The E.Z.N.A.® Total RNA Midi Kit is designed for RNA isolation from large amounts of sample. The E.Z.N.A.® Total RNA Midi Kit can purify total RNA from up to 1×108 cultured cells or 200 mg animal tissues. By using the HiBind® midi column technology, the E.Z.N.A.® Total RNA Midi Kit greatly reduces processing time when compared to traditional methods such as CsCl ultracentrifugation or phenol/chloroform protocols. Purified RNA can be used in downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||250-500 µL|
|Starting Material||Cell and tissue|
|Starting Amount||1×108 cultured cells or 200 mg animal tissues|
|RNA Yield||Up to 600 µg|
|Processing Mode||Manual, centrifugation/vacuum|
|Throughput||1 - 24|
|RNA Binding Technology||Silica Midi spin column|
|Binding Capacity||600 µg|
Protocol and Resources
Product Documentation & Literature