The E.Z.N.A.® Total RNA Kit I provides a straightforward and rapid method for the isolation of up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues. Multiple samples of up to 1 x 107 eukaryotic cells or 30 mg of tissue can be processed in parallel in fewer than 20 minutes. Purified RNA can be used in many downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
- Rapid – 20 minutes or less
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High quality – Purified RNA suitable for a variety of downstream applications
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||40-70 μL|
|Starting Material||Cultured eukaryotic cells and soft tissues|
|Starting Amount||< 1 x 107 cells or 30 mg tissue|
|RNA Yield||Up to 100 µg|
|Processing Mode||Manual, centrifugation/vacuum|
|Throughput||1 - 24|
|RNA Binding Technology||Silica Mini spin column|
|Binding Capacity||100 µg|
Protocol and Resources
Product Documentation & Literature
Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.
Figure 1. Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.
RNA yields from HEK-293 cells using E.Z.N.A.® Total RNA Kit I.
Figure 2. Total RNA was isolated from 2.5×106 HEK-293 cultured cells with Omega Bio-tek’s E.Z.N.A.® Total RNA Kit I with an elution volume of 50 µL. RNA concentrations were quantified with Thermo Scientific’s NanoDrop® 2000c. RNA quality was assessed with the Agilent TapeStation® 2200.
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