The E.Z.N.A.® Stool DNA Kit allows rapid and reliable isolation of high-quality total DNA from fresh and frozen stool samples. Up to 200 mg of stool samples can be processed in less than 60 minutes. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of spin column technology to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from stool samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization technique.
- Reliable – Reproducible DNA purification from a variety of sample sources
- High-quality – Inhibitor removal technology provides efficient PCR inhibitor removal
- High yield – Efficient purification of DNA from even specialized samples
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||Up to 200 mg|
|Elution Volume||50-100 μL|
|Technology||HiBind® DNA Mini Column|
Protocol and Resources
Product Documentation & Literature
Genomic DNA purified using the E.Z.N.A. Stool DNA Kit.
Figure 1. Purified genomic DNA from 7 different 0.1 g stool samples was extracted with the E.Z.N.A.® Stool DNA Kit. Genomic DNA (10% of total purified DNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA. M: Lambda Hind III DNA Marker.
Amplification of genomic DNA isolated from various stool samples using the E.Z.N.A.® Stool DNA Kit
Figure 2. A total of 2 µL of each eluate was amplified with a PCR mastermix and bacterial 16S RNA primers 225 bp to determine whether inhibitors were present in the eluted DNA. Each lane contains 10% of PCR product separated on a 1.5% agarose gel. M: CL5000 DNA Marker. N: Negative control.
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Hongwei Qi, Zhentian Xiang, Guoquan Han, Bing Yu, Zhiqing Huang, Daiwen Chen
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