The E.Z.N.A.® SQ Total RNA Kit is designed for isolating total RNA from animal, tissue and cultured cells. This solution-based system can be easily scaled up and down based on the starting material. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation that are eliminated. RNA purified using the E.Z.N.A.® SQ Total RNA method can be directly used for applications such as RT-PCR*, Northern blotting, and other enzymatic reactions.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Starting Material||Animal tissue and cultured cells|
|Processing Mode||Manual, centrifugation|
|Throughput||1 - 24|
|RNA Binding Technology||Precipitation|
Protocol and Resources
Product Documentation & Literature
RNA yield purified from rice using E.Z.N.A.® SQ Total RNA Kit
Figure 1. Yield of total RNA purified using the E.Z.N.A.® SQ Total RNA Kit. Purified total RNA from 50 mg rice leaf was isolated with the E.Z.N.A.® SQ Total RNA Kit. RNA was eluted in 30 µL DEPC-treated water and quantitated using Thermo Fisher’s NanoDrop® 2000.