The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen, and anti-coagulated whole blood. The method can also be used for the preparation of genomic DNA from buffy coat, bone marrow or cultured cells. The kit allows single or multiple, simultaneous processing of samples in under 90 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ Blood DNA method is ready for applications such as PCR*, Southern blotting, and restriction digestion.
- Isolation of high molecular weigh genomic DNA
- Versatile starting amount – From 0.1 -10 mL
- Rapid – Less than 90 minutes
For Research Use Only. Not for use in diagnostic procedures.
|Starting Material||Fresh and frozen anticoagulated whole blood, body fluid, buccal swab, dried blood, buffy coat|
|Note||gDNA, mDNA, viral DNA|
Protocol and Resources
Product Documentation & Literature
Genomic DNA yield from 200 µL whole blood using E.Z.N.A.® SQ Blood DNA Kit
Figure 1. Genomic DNA yield from 200 µL whole blood sample. Genomic DNA was isolated fron 7 200 µL whole blood samples stored in EDTA using the SQ Blood DNA Kit. O.D. ratio and yield was calculating using a Thermo NanoDrop® 2000.