E.Z.N.A.® Plasmid DNA Mini Kit II

$0.00$301.90

  • Rapid – Purification of plasmid DNA in 30 minutes or less
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for downstream applications

This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid DNA Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream ApplicationCloning; sanger sequencing etc.
Starting material5-15 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual, centrifugation or vacuum
Throughput1 – 24
DNA binding technologySilica Mini Spin Column II (2x capacity of mini spin column)
Lysate clearance methodCentrifugation
Processing time<30 minutes
Yield40-70 µg for high copy-number; 1-15 µg for low copy-number

Product Information

Document NameLanguagesLink
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
Elution BufferREACHGreekgreekreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHItalianitalianreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFinnishfinnishreach
HBC BufferGHSEnglishenglishghs
HBC BufferWHMSFrenchfrenchwhms
HBC BufferWHMSEnglishenglishwhms
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHGermangermanreach
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHFinnishfinnishreach
RNase AGHSSpanishspanishghs
RNase AWHMSFrenchfrenchwhms
RNase AWHMSEnglishenglishwhms
RNase AREACHSwedishswedishreach
RNase AREACHSpanishspanishreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHGermangermanreach
RNase AGHSEnglishenglishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFrenchfrenchreach
RNase AREACHItalianitalianreach
RNase AREACHFinnishfinnishreach
Solution IGHSSpanishspanishghs
Solution IWHMSFrenchfrenchwhms
Solution IWHMSEnglishenglishwhms
Solution IREACHSwedishswedishreach
Solution IREACHSpanishspanishreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHGermangermanreach
Solution IGHSEnglishenglishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFrenchfrenchreach
Solution IREACHItalianitalianreach
Solution IREACHFinnishfinnishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSwedishswedishreach
Solution IIREACHItalianitalianreach
Solution IIREACHDanishdanishreach
Solution IIREACHSpanishspanishreach
Solution IIGHSSpanishspanishghs
Solution IIREACHFrenchfrenchreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
Solution IIREACHEnglishenglishreach
Solution IIGHSEnglishenglishghs
Solution IIREACHGermangermanreach
Solution IIIREACHEnglishenglishreach
Solution IIIREACHDanishdanishreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHGermangermanreach
Solution IIIREACHItalianitalianreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHSwedishswedishreach
Solution IIIWHMSEnglishenglishwhms
Solution IIIWHMSFrenchfrenchwhms
Solution IIIGHSEnglishenglishghs
Solution IIIGHSSpanishspanishghs
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