The E.Z.N.A.® Plasmid DNA Midi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiBind® DNA columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be processed simultaneously. Up to 100-250 µg high copy number plasmid DNA or 10-50 µg low copy number plasmid DNA can be purified from 20-50 mL overnight culture using the E.Z.N.A.® Plasmid DNA Midi Kit. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations.
For Research Use Only. Not for use in diagnostic procedures.
FEATURES | SPECIFICATIONS |
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Downstream Application | Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. |
Starting material | 15-50 mL LB culture; Optimal Cell Mass (OD600 x mL culture) is 80-100. |
Plasmid type | High-copy, low-copy, cosmid DNA |
Processing mode | Manual (centrifugation or vacuum) |
Throughput | 1 – 24 |
DNA binding technology | Silica Midi Spin Column |
Lysate clearance method | Centrifugation |
Processing time | <60 minutes |
Yield | 100-250 µg for high copy-number; 10-50 µg for low copy-number |
ITEM | AVAILABLE SEPARATELY |
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HiBind® DNA Mini Columns | View Product |
15 mL Collection Tubes | — |
Solution I | View Product |
Solution II | View Product |
Solution III | View Product |
HBC Buffer | View Product |
DNA Wash Buffer | View Product |
RNase A | View Product |
Elution Buffer | View Product |
Table 1. pGEM plasmid was purified from 50 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Midi Kit following manufacturer’s recommended protocol. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.
Product Information
Safety Data Sheets
Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
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DNA Wash Buffer | GHS | Spanish | spanish | ghs | |
DNA Wash Buffer | GHS | English | english | ghs | |
DNA Wash Buffer | WHMS | French | french | whms | |
DNA Wash Buffer | WHMS | English | english | whms | |
DNA Wash Buffer | REACH | Swedish | swedish | reach | |
DNA Wash Buffer | REACH | Spanish | spanish | reach | |
DNA Wash Buffer | REACH | Norwegian | norwegian | reach | |
DNA Wash Buffer | REACH | Italian | italian | reach | |
DNA Wash Buffer | REACH | German | german | reach | |
DNA Wash Buffer | REACH | French | french | reach | |
DNA Wash Buffer | REACH | Finnish | finnish | reach | |
DNA Wash Buffer | REACH | Danish | danish | reach | |
DNA Wash Buffer | REACH | English | english | reach | |
Elution Buffer | REACH | Greek | greek | reach | |
Elution Buffer | REACH | Portuguese | portuguese | reach | |
Elution Buffer | REACH | Hungarian | hungarian | reach | |
Elution Buffer | REACH | Dutch | dutch | reach | |
Elution Buffer | WHMS | French | french | whms | |
Elution Buffer | WHMS | English | english | whms | |
Elution Buffer | REACH | Swedish | swedish | reach | |
Elution Buffer | REACH | Spanish | spanish | reach | |
Elution Buffer | REACH | Italian | italian | reach | |
Elution Buffer | GHS | English | english | ghs | |
Elution Buffer | GHS | Spanish | spanish | ghs | |
Elution Buffer | REACH | English | english | reach | |
Elution Buffer | REACH | Danish | danish | reach | |
Elution Buffer | REACH | French | french | reach | |
Elution Buffer | REACH | Norwegian | norwegian | reach | |
Elution Buffer | REACH | German | german | reach | |
Elution Buffer | REACH | Finnish | finnish | reach | |
HBC Buffer | GHS | English | english | ghs | |
HBC Buffer | WHMS | French | french | whms | |
HBC Buffer | WHMS | English | english | whms | |
HBC Buffer | REACH | Swedish | swedish | reach | |
HBC Buffer | REACH | Spanish | spanish | reach | |
HBC Buffer | REACH | Norwegian | norwegian | reach | |
HBC Buffer | REACH | German | german | reach | |
HBC Buffer | GHS | Spanish | spanish | ghs | |
HBC Buffer | REACH | English | english | reach | |
HBC Buffer | REACH | Danish | danish | reach | |
HBC Buffer | REACH | French | french | reach | |
HBC Buffer | REACH | Italian | italian | reach | |
HBC Buffer | REACH | Finnish | finnish | reach | |
RNase A | GHS | Spanish | spanish | ghs | |
RNase A | WHMS | French | french | whms | |
RNase A | WHMS | English | english | whms | |
RNase A | REACH | Swedish | swedish | reach | |
RNase A | REACH | Spanish | spanish | reach | |
RNase A | REACH | Norwegian | norwegian | reach | |
RNase A | REACH | German | german | reach | |
RNase A | GHS | English | english | ghs | |
RNase A | REACH | English | english | reach | |
RNase A | REACH | Danish | danish | reach | |
RNase A | REACH | French | french | reach | |
RNase A | REACH | Italian | italian | reach | |
RNase A | REACH | Finnish | finnish | reach | |
Solution I | GHS | Spanish | spanish | ghs | |
Solution I | WHMS | French | french | whms | |
Solution I | WHMS | English | english | whms | |
Solution I | REACH | Swedish | swedish | reach | |
Solution I | REACH | Spanish | spanish | reach | |
Solution I | REACH | Norwegian | norwegian | reach | |
Solution I | REACH | German | german | reach | |
Solution I | GHS | English | english | ghs | |
Solution I | REACH | English | english | reach | |
Solution I | REACH | Danish | danish | reach | |
Solution I | REACH | French | french | reach | |
Solution I | REACH | Italian | italian | reach | |
Solution I | REACH | Finnish | finnish | reach | |
Solution II | REACH | Finnish | finnish | reach | |
Solution II | REACH | Norwegian | norwegian | reach | |
Solution II | REACH | Swedish | swedish | reach | |
Solution II | REACH | Italian | italian | reach | |
Solution II | REACH | Danish | danish | reach | |
Solution II | REACH | Spanish | spanish | reach | |
Solution II | GHS | Spanish | spanish | ghs | |
Solution II | REACH | French | french | reach | |
Solution II | WHMS | English | english | whms | |
Solution II | WHMS | French | french | whms | |
Solution II | REACH | English | english | reach | |
Solution II | GHS | English | english | ghs | |
Solution II | REACH | German | german | reach | |
Solution III | REACH | English | english | reach | |
Solution III | REACH | Danish | danish | reach | |
Solution III | REACH | Finnish | finnish | reach | |
Solution III | REACH | French | french | reach | |
Solution III | REACH | German | german | reach | |
Solution III | REACH | Italian | italian | reach | |
Solution III | REACH | Norwegian | norwegian | reach | |
Solution III | REACH | Spanish | spanish | reach | |
Solution III | REACH | Swedish | swedish | reach | |
Solution III | WHMS | English | english | whms | |
Solution III | WHMS | French | french | whms | |
Solution III | GHS | English | english | ghs | |
Solution III | GHS | Spanish | spanish | ghs |