The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi Column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50-200 mL of overnight culture in LB medium typically produces 0.5-1 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription in vitro, and other applications.
- Speed – Purification of plasmid DNA in < 60 minutes
- Safety – No phenol/chloroform extractions
- Yield – Up to 1 mg of plasmid DNA
- High quality – DNA is suitable for downstream applications
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||Cloning; sanger sequencing etc.|
|Starting material||50-200 mL LB culture with OD600 between 2 and 3; or equivalent|
|Plasmid type||High-copy, low-copy, cosmid DNA|
|Processing mode||Manual, centrifugation or vacuum|
|Throughput||1 - 24|
|DNA binding technology||Silica Maxi Spin Column|
|Lysate clearance method||Centrifugation|
|Processing time||<60 minutes|
|Yield||600-1200 µg for high copy-number; 50-300 µg for low copy-number|
Protocol and Resources
Product Documentation & Literature
Yield and quality of plasmid DNA purified using E.Z.N.A.® Plasmid Midi and Maxi Kits
Figure 1. Bacterial cultures grown for 24 hours in LB broth were used in respective culture amounts. DH5α cells transformed with pGEM vector. O.D. calculated using Thermo Scientific’s NanoDrop® 2000c.
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