E.Z.N.A.® Plant & Fungal RNA Kit
Price range: $0.00 through $1,111.30
Isolate total RNA from plant and fungal samples using spin columns
The E.Z.N.A.® RNA family of products is an innovative system that radically simplifies the extraction and purification of RNA from a variety of sources. The key to this system is that it uses the reversible binding properties of the HiBind® matrix (a silica-based material) in combination with the speed of mini column spin technology. Single or multiple samples can be processed quickly. There is no need for phenol/chloroform extractions and time-consuming steps, such as CsCl gradient ultra-centrifugation and precipitation with isopropanol or LiCL, are eliminated.
RNA purified using the E.Z.N.A.® RNA purification system is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
The E.Z.N.A.® Plant & Fungal RNA Kit can purify up to 100 µg plant or fungal RNA that is >200 nt. Normally, 10-100 mg plant or fungal tissue can be processed in a single experiment. Lysis of cells or tissue occurs under denaturing conditions that inactivate RNases. After the homogenization process, samples are transferred to the HiBind® RNA Mini Column to bind RNA. Cellular debris and other contaminants are removed after three quick wash steps. High-quality RNA is eluted in sterile Nuclease-free Water.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 50-100 μL |
| Starting Material | Plant tissue |
| Starting Amount | <100 mg |
| RNA Yield | Up to 100 µg, >200 nt |
| Processing Mode | Spin column |
| Throughput | 1 – 24 |
| RNA Binding Technology | Silica technology |
| Binding Capacity | 100 µg |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® RNA Mini Columns | — |
| 2 mL Collection Tubes | View Product |
| Homogenizer Mini Columns | — |
| NTL Lysis Buffer | — |
| SP Buffer | — |
| RB Buffer | — |
| RNA Wash Buffer I | View Product |
| RNA Wash Buffer II | View Product |
| Nuclease-free Water | — |
Figure 1. Omega Bio-tek’s yield vs. Company Q’s yield. Total RNA was isolated from 100 mg of Arabidopsis thaliana leaf tissue according to manufacturer’s recommended protocols. One tenth of eluate was analyzed on a denaturing agent agarose gel. O.D. values calculated with Thermo Scientific’s NanoDrop® 2000c.
Reproducible RNA Yield and Purity
Figure 2. Total RNA was isolated from 20 mg fresh canola using E.Z.N.A.® Plant RNA Kit and eluted in 100 µL. RNA concentrations were quantified via Thermo Scientific’s NanoDrop® 2000c. A260/A280 ratios were approximately 2.0 suggesting high purity for RNA.
Intact RNA from Tough Plant Samples
Figure #. Total RNA was isolated from 20 mg fresh canola using E.Z.N.A.® Plant RNA Kit and eluted in 100 µL. RNA quality was assessed via the Agilent TapeStation® 2200.