E.Z.N.A.® Plant RNA Kit

Overview

E.Z.N.A.® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogenizer column for filtration and homogenization of viscous plant cell lysate by centrifugation in combination with the HiBind RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, Northern blot analysis, differential display, and poly A+ RNA selection.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Features	Specifications
Downstream Application	PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume	50-100 μL
Starting Material	Plant tissue
Starting Amount	<100 mg
RNA Yield	Up to 100 µg, >200 nt
Processing Mode	Spin column
Throughput	1 - 24
RNA Binding Technology	Silica technology
Binding Capacity	100 µg

Protocol and Resources

Product Documentation & Literature

Product Data

Comparison of yield and quality between E.Z.N.A.® Plant RNA Kit and a leading competitor.

Figure 1. Omega Bio-tek’s yield vs. Company Q’s yield. Total RNA was isolated from 100 mg of Arabidopsis thaliana leaf tissue according to manufacturer’s recommended protocols. One tenth of eluate was analyzed on a denaturing agent agarose gel. O.D. values calculated with Thermo Scientific’s NanoDrop® 2000c.

Samples validated with E.Z.N.A.® Plant RNA Kit

• Arabidopis thaliana
• Humulus lupulus
• Beta vulgaris
• Fragraria virginia
• Clarkia spp.
• Daucus carota
• Ornithogalum thyrsoides
• Dendranthema spp.
• Euglena graciis
• Oryza sativa
• Hordeum vulgare
• Lycopersicon esculentum
• Malus sp.
• Solum tuberosum
• Spinacia oleracaea
• Surfinia sp.
• Triticum asestivum
• Vetis sp.
• Zea mays
• Nicotiana tabacum

Publications