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E.Z.N.A.® Plant RNA Kit
Isolate total RNA from plant samples using spin columns
Overview
E.Z.N.A.® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogenizer column for filtration and homogenization of viscous plant cell lysate by centrifugation in combination with the HiBind RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, Northern blot analysis, differential display, and poly A+ RNA selection.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 50-100 μL |
| Starting Material | Plant tissue |
| Starting Amount | <100 mg |
| RNA Yield | Up to 100 µg, >200 nt |
| Processing Mode | Spin column |
| Throughput | 1 - 24 |
| RNA Binding Technology | Silica technology |
| Binding Capacity | 100 µg |
Protocol and Resources
Product Documentation & Literature
SALES SHEET
Product Data
Comparison of yield and quality between E.Z.N.A.® Plant RNA Kit and a leading competitor.
Figure 1. Omega Bio-tek’s yield vs. Company Q’s yield. Total RNA was isolated from 100 mg of Arabidopsis thaliana leaf tissue according to manufacturer’s recommended protocols. One tenth of eluate was analyzed on a denaturing agent agarose gel. O.D. values calculated with Thermo Scientific’s NanoDrop® 2000c.
- Arabidopis thaliana
- Humulus lupulus
- Beta vulgaris
- Fragraria virginia
- Clarkia spp.
- Daucus carota
- Ornithogalum thyrsoides
- Dendranthema spp.
- Euglena graciis
- Oryza sativa
- Hordeum vulgare
- Lycopersicon esculentum
- Malus sp.
- Solum tuberosum
- Spinacia oleracaea
- Surfinia sp.
- Triticum asestivum
- Vetis sp.
- Zea mays
- Nicotiana tabacum
Publications
View Publications
- Overexpression of ALDH2B8, an aldehyde dehydrogenase gene from grapevine, sustains Arabdiopsis growth upon salt stress and protects plants against oxidative stress
Xiaohao Xu, Rongrong Guo, Chenxia Cheng, Hongjing Zhang, Yucheng Zhang, Xipeng Wang
| Format | Miniprep |
|---|
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