E.Z.N.A.® MicroElute Genomic DNA Kit provides a rapid and easy method for the isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results. Using the reversible binding properties of a silica-based material, the speed of mini-column spin technology and specifically formulated buffers, the system allows the purification of genomic DNA up to 60 kb. Samples are first lysed under denaturing conditions and then applied to the HiBind MicroElute® Column to bind DNA, while cellular debris, hemoglobin, and other proteins are effectively washed away. High-quality DNA can be eluted with 10-15 µL sterile deionized water or Elution Buffer from the MicroElute® column.
- Purify genomic DNA from small sample volumes and a small amount of cells
- High-quality genomic DNA purification from a variety of samples
- No phenol/chloroform extractions
- Purified DNA suitable for most downstream applications
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||10 mg tissue|
|Starting Material||Tissue, blood serum body fluids dried blood semen swab forensic samples|
|Elution Volume||10-50 µL|
|Technology||MicroElute LE DNA Column|
Protocol and Resources
Product Documentation & Literature
Genomic DNA was successfully purified from mouse tip snips using E.Z.N.A.® MicroElute gDNA Kit.
Figure 1. Genomic DNA purified using the E.Z.N.A.® MicroElute Genomci DNA Kit. Purified genomic DNA from mouse tail snips was isolated with the E.Z.N.A.® MicroElute Genomic DNA Kit. Genomic DNA was extracted from 10 mg mouse tail and 5% of each eluate DNA was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA.