The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind® matrix that specifically, but reversibly, binds DNA or RNA under
optimized conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or a low salt buffer. The MicroElute® Clean Up system, designed for rapid DNA clean up, includes:
• MicroElute® Cycle-Pure – for direct purification of double or single stranded PCR products (100 bp – 10 kb) from amplification reactions
• MicroElute® Gel Extraction – for extraction of DNA fragments from standard, or low-melt agarose gels in TAE (Tris-acetate/EDTA) or TBE (Tris-borate/EDTA) buffer
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 10-15 µL |
| Starting material | Agrose gel slice |
| Starting amount | Up to 10 µg DNA |
| DNA recovered | >90% recovery, 70 bp to 20 kb |
| Processing mode | Manual, centrifugation/vacuum |
| Throughput | 1 |
| DNA binding technology | Silica MicroElute spin column |
| Binding capacity | 10 μg |
| Processing time | <15 minutes |
| Special note | Low elution volume |

