The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation|
|Elution Volume||15-30 μL for miRNA; 40-70 μL for >200 nt|
|Starting Material|| Cultured eukaryotic cells or bacteria, or from animal,
plant, or fungal tissues
|Starting Amount||Up to 100 mg|
|RNA Yield||Up to 50 µg|
|Processing Mode||Spin column|
|Throughput||1 - 24|
|RNA Binding Technology||Silica technology|
|Binding Capacity||100 µg for each|
Protocol and Resources
Product Documentation & Literature
MicroRNA was purified using the E.Z.N.A.® Micro RNA Kit
Figure 1. Purified large RNAs and miRNA from tissue samples was isolated with the E.Z.N.A.® miRNA Isolation Kit. RNA was extracted from samples as follows: sample 1 from fish liver, sample 2 from frog liver. Large RNA (5% of total purified RNA) was analyzed on a 1% agarose gel. miRNA (5% of total purified RNA) was analyzed on a 3% agarose gel to demonstrate yield and quality of the RNA.
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