The E.Z.N.A.® High Performance (HP) Fungal DNA Kit is designed for efficient recovery of genomic DNA from fresh and dried fungal tissue samples rich in polysaccharides or having lower DNA content. Up to 100 mg wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin-column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||100 mg wet tissue (or 30 mg dry tissue)|
|Starting Material||Fresh and dried fungal tissue samples rich in polysaccharides or having lower DNA content|
|Elution Volume||50-100 μL|
|Technology||HiBind® DNA Mini Column|
Protocol and Resources
Product Documentation & Literature
Total cellular DNA was successfully purified from various fungal samples using E.Z.N.A.® HP Fungal DNA Kit.
Figure 1. Genomic DNA purified using the E.Z.N.A.® HP Fungal DNA Kit. Purified genomic DNA from various fungi was isolated with the E.Z.N.A.® HP Fungal DNA Kit. DNA was extracted from 100 mg fresh fungal tissue as follows: lane 1 from Ganoderma lucidum, lane 2 from Cardyceps sinesis, lane 3 from Agaricus campetris, lane 4 from Russula foetens. Genomic DNA (200 ng of total purified DNA) was analyzed on a 0.8% agarose gel to demonstrate yield and quality of the DNA. M: Lambda Hind III DNA Marker.