E.Z.N.A.® Fungal RNA Mini Kit

Overview

Modified from the E.Z.N.A.® Plant RNA Kit, the E.Z.N.A.® Fungal RNA Kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind ® RNA spin columns, this method permits purification of high-quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, Northern Blotting, Poly A +(mRNA) purification, and differential display.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Features	Specifications
Downstream Application	PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume	50-100 μL
Starting Material	Fungal Tissue
Starting Amount	<250 mg
RNA Yield	Up to 100 µg, >200 nt
Processing Mode	Spin column
Throughput	1 - 24
RNA Binding Technology	1 - 24
Binding Capacity	100 µg

Protocol and Resources

Product Documentation & Literature

Product Data

Total RNA purified from various fungal tissues using E.Z.N.A.® Fungal RNA Kit.

Figure 1. Total RNA purified using the E.Z.N.A.® Fungal RNA Kit. Purified total RNA from 100 mg fungal species was isolated with the E.Z.N.A.® Fungal RNA Kit. RNA was extracted from fungal species as follows: sample 1 from E. edodes, sample 2 from V. volvacea, sample 3 from F. velutipes, sample 4 from H. erinaceum. Total RNA (10% of total purified RNA) was analyzed to demonstrate yield and quality of the RNA.

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