Modified from the E.Z.N.A.® Plant RNA Kit, the E.Z.N.A.® Fungal RNA Kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind ® RNA spin columns, this method permits purification of high-quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, Northern Blotting, Poly A +(mRNA) purification, and differential display.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||50-100 μL|
|Starting Material||Fungal Tissue|
|Starting Amount||<250 mg|
|RNA Yield||Up to 100 µg, >200 nt|
|Processing Mode||Spin column|
|Throughput||1 - 24|
|RNA Binding Technology||1 - 24|
|Binding Capacity||100 µg|
Protocol and Resources
Product Documentation & Literature
Total RNA purified from various fungal tissues using E.Z.N.A.® Fungal RNA Kit.
Figure 1. Total RNA purified using the E.Z.N.A.® Fungal RNA Kit. Purified total RNA from 100 mg fungal species was isolated with the E.Z.N.A.® Fungal RNA Kit. RNA was extracted from fungal species as follows: sample 1 from E. edodes, sample 2 from V. volvacea, sample 3 from F. velutipes, sample 4 from H. erinaceum. Total RNA (10% of total purified RNA) was analyzed to demonstrate yield and quality of the RNA.
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