The E.Z.N.A.® DNA/RNA Isolation Kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenized in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind® RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, the maximum yield of DNA and RNA can be purified from the entire sample.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||40-70 µL for RNA; 50-100 µL for DNA|
|Starting Material||Cultured cells and animal tissues|
|Starting Amount||Up to 1X107 cells; up to 30 mg tissue|
|RNA Yield||RNA in one fraction; DNA in another|
|Processing Mode||Manual, centrifugation|
|Throughput||1 - 24|
|RNA Binding Technology||Silica Mini spin column|
|Binding Capacity||100 µg for DNA and RNA|
Protocol and Resources
Product Documentation & Literature
Genomic DNA and total RNA purified using the E.Z.N.A.® DNA/RNA Isolation Kit.
Figure 1. DNA/RNA was extracted from tissue samples. DNA (5% of total purified DNA) was analyzed on a 0.8% agarose gel. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA/RNA. Sample 1 from mouse liver, sample 2 from mouse kidney, sample 3 from mouse spleen, sample 4 from HeLa cells, and sample 5 from cos-7 cells.