E.Z.N.A.® Cycle Pure Kit (V-spin)
$0.00 – $666.30
- Rapid – Purification of PCR products in less than 10 minutes
- Safe – No Phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for a variety of downstream applications
The E.Z.N.A.® Cycle Pure Kit is a convenient system for the fast and reliable purification of PCR products. The E.Z.N.A.® Cycle Pure Kit uses HiBind® technology to recover DNA bands from 100 bp to 10 kb free of oligonucleotides, nucleotides, and polymerase with yields exceeding 80%. The binding conditions of the HiBind® DNA Mini Columns are adjusted by the addition of a specially formulated buffer before adding the sample. Following a rapid wash step, DNA is eluted with deionized water or a low salt buffer. Purified DNA can be directly used for most downstream applications include T-A ligations, PCR sequencing, restriction enzyme digestion, or various labeling reactions.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream application | T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions |
| Elution volume | 30-50 µL |
| Starting material | ssDNA, dsDNA, PCR products |
| Starting amount | 5-50 µL |
| DNA recovered | >80% recovery, 100 bp to 10 kb |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1-24 |
| DNA binding technology | Silica mini spin column |
| Processing time | <10 minutes |
| Special Notes | For clean-up of DNA fragments < 200 bp, please follow the protocol modification outlined in the product manual |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® DNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| CP Buffer | View Product |
| Elution Buffer | View Product |
| DNA Wash Buffer | View Product |
Gel Electrophoresis Analysis
Figure 1. 500 bp and 5 kb DNA fragments were cleaned up with products from Company T, Company A, Company P, Company Q and Omega Bio-tek following manufaturer’s recommended protocols. DNA was analyzed on a 0.8% agarose gel.
Sanger Sequencing
Figure 2. 500 bp amplicon was purified with the E.Z.N.A. Cycle Pure Kit was used in a 5 µL Sanger sequencing reaction. DNA was analyzed on an Applied Biosystem 3730XL.
FAQs
What are the minimum and maximum size products that can be isolated with the E.Z.N.A. Cycle Pure Kit?
The E.Z.N.A. Cycle Pure Kit can bind DNA between 100 bp – 10 kbp. The protocol mentions, “for PCR products smaller than 200 bp, add 5 volumes CP Buffer and 0.4 volumes 100% isopropanol”. If you follow this modification, the minimum bp size that the columns would bind would be 50bp.
What is the binding capacity of the column in D6492?
30 µg
Product Information
Safety Data Sheets
| Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
|---|---|---|---|---|---|
| CP Buffer | GHS | Spanish | spanish | ghs | |
| CP Buffer | GHS | English | english | ghs | |
| CP Buffer | WHMS | French | french | whms | |
| CP Buffer | WHMS | English | english | whms | |
| CP Buffer | REACH | Swedish | swedish | reach | |
| CP Buffer | REACH | Spanish | spanish | reach | |
| CP Buffer | REACH | Norwegian | norwegian | reach | |
| CP Buffer | REACH | Italian | italian | reach | |
| CP Buffer | REACH | German | german | reach | |
| CP Buffer | REACH | French | french | reach | |
| CP Buffer | REACH | Finnish | finnish | reach | |
| CP Buffer | REACH | Danish | danish | reach | |
| CP Buffer | REACH | English | english | reach | |
| DNA Wash Buffer | WHMS | French | french | whms | |
| DNA Wash Buffer | WHMS | English | english | whms | |
| DNA Wash Buffer | REACH | Swedish | swedish | reach | |
| DNA Wash Buffer | REACH | Spanish | spanish | reach | |
| DNA Wash Buffer | REACH | Norwegian | norwegian | reach | |
| DNA Wash Buffer | REACH | Italian | italian | reach | |
| DNA Wash Buffer | REACH | French | french | reach | |
| DNA Wash Buffer | REACH | Finnish | finnish | reach | |
| DNA Wash Buffer | REACH | Danish | danish | reach | |
| DNA Wash Buffer | REACH | English | english | reach | |
| DNA Wash Buffer | GHS | Spanish | spanish | ghs | |
| DNA Wash Buffer | GHS | English | english | ghs | |
| DNA Wash Buffer | REACH | German | german | reach | |
| Elution Buffer | REACH | Greek | greek | reach | |
| Elution Buffer | REACH | Portuguese | portuguese | reach | |
| Elution Buffer | REACH | Hungarian | hungarian | reach | |
| Elution Buffer | REACH | Dutch | dutch | reach | |
| Elution Buffer | WHMS | French | french | whms | |
| Elution Buffer | WHMS | English | english | whms | |
| Elution Buffer | REACH | Swedish | swedish | reach | |
| Elution Buffer | REACH | Spanish | spanish | reach | |
| Elution Buffer | REACH | Norwegian | norwegian | reach | |
| Elution Buffer | REACH | Italian | italian | reach | |
| Elution Buffer | REACH | German | german | reach | |
| Elution Buffer | REACH | French | french | reach | |
| Elution Buffer | REACH | Finnish | finnish | reach | |
| Elution Buffer | REACH | Danish | danish | reach | |
| Elution Buffer | REACH | English | english | reach | |
| Elution Buffer | GHS | Spanish | spanish | ghs | |
| Elution Buffer | GHS | English | english | ghs |
