The E.Z.N.A.® Circulating DNA Kit provides a rapid and easy method for the isolation of circulating DNA from plasma, serum, and other acellular body fluids. The kit allows for simultaneous processing of single or multiple samples in under 120 minutes. DNA purified using the E.Z.N.A.® Circulating DNA method is ready for applications such as PCR, microarrays, and next generation sequencing.
E.Z.N.A.® Circulating DNA Kit uses the reversible nucleic acid binding properties of our HiBind® matrix combined with the speed of mini column centrifugation and funnel adaptors. The funnel adaptors allow for large volumes of liquid to be applied to the mini columns when using a vacuum manifold.
A specially formulated buffer system allows circulating DNA to bind to the HiBind® matrix. Samples are lysed under denaturing conditions and then transferred to the DNA column where DNA binds and cellular debris, hemoglobin, and other proteins are washed away. High-quality DNA is eluted in nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||1-4 mL cell-free fluid|
|Starting Material||Plasma, serum, and other acellular body fluids|
|Elution Volume||30-150 μL|
|Technology||HiBind® DNA Mini Column|
Protocol and Resources
Product Documentation & Literature
Circulating cfDNA purified by E.Z.N.A.® Circulating DNA Kit has excellent yield and minimal genomic DNA contamination.
Figure 1. Electropherogram overlay of purified DNA from 4 mL serum. 4 mL of unspiked serum was purified using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. Purified DNA was analyzed on Agilent’s TapeStation® 2200.
E.Z.N.A.® Circulating DNA Kit uses less volumes of lysis/binding buffers and the whole process takes less time than the leading competitor.