The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 mL of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNase’s, the lysate is homogenized with a homogenizer spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as hemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC-treated water.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||50-100 μL|
|Starting Material||Whole blood; animal tissue; others with modified protocols|
|Starting Amount||Up to 1 mL blood; up to 30 mg tissue|
|RNA Yield||Up to 100 µg|
|Processing Mode||manual, centrifugation|
|Throughput||1 - 24|
|RNA Binding Technology||Silica Mini spin column|
|Binding Capacity||100 µg|
Protocol and Resources
Product Documentation & Literature
Total RNA purified using E.Z.N.A.® Blood RNA Kit.
Figure 1. Total RNA purified using the E.Z.N.A.® Blood RNA Kit. Purified total RNA from various blood was isolated with the E.Z.N.A.® Blood RNA Kit. RNA was extracted from blood samples as follows: sample 1 from 200 µL human blood, sample 2 from 400 µL human blood, sample 3 from 1 mL human blood, sample 4 from 100 µL mouse blood, and sample 5 from 200 µL mouse blood. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.