E.Z.N.A.® Blood DNA Mini Kit
Price range: $371.00 through $1,013.50
Isolate DNA from 1-250 µL whole blood using spin columns.
The E.Z.N.A.® Tissue DNA Kit is the recommended alternative.
The E.Z.N.A.® Blood DNA Kit provides rapid total DNA isolation from 1-250 µL of fresh and frozen anticoagulated whole blood. The E.Z.N.A.® Blood DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® Blood DNA method is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 1-250 µL |
| Starting Material | Fresh and frozen anticoagulated whole blood, body fluid, buccal swab, dried blood, buffy coat |
| Yield | 100 µg |
| Elution Volume | 100-200 μL |
| Technology | HiBind® DNA Mini Column |
| Processing Mode | Manual, centrifugation/vacuum |
| Throughput | 1-24 |
Yield & Quality
Figure 1. Genomic DNA isolated using the E.Z.N.A.® Blood DNA Mini Kit. DNA was purified from 4 mL saliva stored in Oragene® tubes using the E.Z.N.A.® Blood DNA Mini Kit. DNA was eluted in 500 µL and quantitated using the Thermo Scientific NanoDrop® 2000 spectrometer.
gDNA from Human Whole Blood
Table 1. Yield of genomic DNA purified using the E.Z.N.A.® Blood DNA Mini Kit. DNA was purified from packed blood cells of 1 mL whole blood using the E.Z.N.A.® Blood DNA Mini Kit. DNA was eluted in 500 µL and quantitated using the Thermo Scientific NanoDrop® 2000.
High DNA Yield and Purity
Figure 2. Genomic DNA was isolated from 250 µL Whole Human Blood using E.Z.N.A.® Blood DNA Mini Kit and eluedin 100 µL. Purified DNA was quantified via Thermo Scientific’s NanoDrop® 2000c. A260/A280 ratios were approximately ~1.8, suggesting high DNA purity.
High Integrity and Minimal Degradation
Figure 3. Genomic DNA was isolated from 250 µL Whole Human Blood using E.Z.N.A.® Blood DNA Mini Kit with an elution volume of 100 µL. 5 µL of eluate was run on a 1% agarose gel, showing minimal degradation, confirming extraction efficiency and DNA quality for downstream use.
Inhibitor-Free DNA
Figure 4. Genomic DNA was isolated from 250 µL Whole Human Blood using the E.Z.N.A.® Blood DNA Mini Kit and eluted in 100 µL. qPCR results demonstrate an expected ΔCt of ~3.3, reflecting minimal PCR inhibition.