The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large-scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.
- Isolates DNA from samples up to 20 mL
- No organic extractions or alcohol precipitation
- Pure DNA, free of contaminants and enzyme inhibitors
- Entire procedure completion takes less than 1 hour
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||Up to 20 mL|
|Starting Material||Fresh and frozen anticoagulated whole blood, body fluid, buccal swab, dried blood, buffy coat|
|Elution Volume||2000 μL|
|Technology||HiBind® DNA Maxi column|
|Processing Mode||Manual, centrifugation/vaccum|
Protocol and Resources
Product Documentation & Literature
Quality and yield of genomic DNA purified using the E.Z.N.A.® Blood DNA Mini and Maxi Kits.
Figure 1. Purified genomic DNA was isolated from 2 mL human whole blood using the E.Z.N.A.® Blood DNA Midi Kit (samples 1-8) or from 10 mL human whole blood using the E.Z.N.A.® Blood DNA Maxi Kit (samples 9-16). DNA was eluted in 500 µL or 1,000 µL and quantitated using Thermo Fisher’s NanoDrop® 2000.