The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 105 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||50-75 μL|
|Starting Material||Culture cells|
|Starting Amount||up to 5x105 cells|
|Processing Mode||Manual, centrifugation/vacuum|
|Throughput||96 samples per run|
|RNA Binding technology||Silica membrane 96-well|
Protocol and Resources
Product Documentation & Literature
Total purified RNA using the E-Z 96® Total RNA Kit
Figure 1. Purified total RNA from 10 mg chicken liver was isolated with the E-Z 96® Total RNA Kit. Total RNA (500 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.