E-Z 96™ Tissue DNA Kit
Price range: $0.00 through $1,021.20
Isolate DNA from tissues in 96-well plate format
- Rapid – Isolation of DNA from 96 samples in less than 1 hour
- Reliable – Reproducible DNA purification from variety of samples
- Safe – No phenol/chloroform extractions
- Purified DNA suitable for most downstream application
By adapting Hi-Bind technology with 96-well plates, the E-Z 96™ Tissue DNA Kit provides a high-throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridization techniques.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 20 mg |
| Starting Material | Tissue, mouse tail, cultured cells, bacteria, blood |
| Elution Volume | 150 µL |
| Technology | Silica 96-well plate |
| Processing Mode | Manual |
| Throughput | 96 |
Yield & Quality
Figure 1. Genomic DNA purified using the E-Z 96™ Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA.
PCR Analysis
Figure 2. Amplification of genomic DNA isolated from chicken tissue samples using the E-Z 96™ Tissue DNA Kit. DNA was isolated from chicken tissue in duplicate: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. A total of 2 µL of each eluate was amplified with a PCR mastermix and NS gene primers for a 750 bp product to determine whether inhibitors were present in the eluted DNA. Each lane contains 10% of PCR product separated on a 1% agarose gel.
Yield & Quality Across Tissues
Figure 3. Genomic DNA purified using the E-Z 96™ Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA.