Based on the same principle of E.Z.N.A.® Plant RNA Kit, the E-Z 96® Plant RNA kit is designed for isolation of plant RNA in a 96-well plate format. The E-Z 96® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. The kit includes a homogenization plate to efficiently remove cell debris and simultaneously homogenize the lysate. In combination with the HiBind RNA plate, this kit permits purification of high-quality RNA from as much as 40 mg seed tissue or up to 100 mg plant tissue. The system is also efficient enough to allow isolation of total RNA from as little as 0.5 mg tissue. Purified RNA is suitable for RT-PCR, Northern analysis, differential display, and poly A+ RNA selection.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||50-100 μL|
|Starting Material||Plant tissue|
|Starting Amount||<100 mg|
|RNA Yield||up to 100 µg, >200 nt|
|Processing Mode||Spin column|
|Throughput||1 - 24|
|RNA Binding Technology||Silica technology|
|Binding Capacity||100 µg|
Protocol and Resources
Product Documentation & Literature
Total RNA purified from various plant samples using E.Z.N.A.® Plant RNA Kit.
Figure 1. Purified total RNA from 8 of 50 mg plant samples was isolated with the E-Z 96® Plant RNA Kit. RNA was extracted from plant samples as follows: picture 1 from 50 mg maize seeds, picture 2 from 50 mg tomato leaves. Total RNA (10% of total purified RNA) was analyzed on denaturing formaldehyde gel to demonstrate yield and quality of the RNA.