E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek’s SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.
- Rapid – 96 samples DNA isolation in 90 minutes
- Reliable – optimized buffers guarantee pure DNA very time
- Safe – No organic extractions
- High-quality – Purified DNA suitable for most downstream applications
- Automation – E-Z 96® plate is suitable for most robotic workstations
For Research Use Only. Not for use in diagnostic procedures.
|Starting Amount||15 mg dry; 50 mg fresh|
|Starting Material||Plants, such as those with unusually high levels of phenolic compounds or polysaccharides|
|Elution Volume||150 µL|
|Technology||Silica 96-well plate|
Protocol and Resources
Product Documentation & Literature
Total cellular DNA purified using E-Z 96® Plant DNA Kits.
Figure 1. Total cellular DNA purified using the E-Z 96® Plant DNA Kit. Purified cellular DNA from various plant sources was isolated with the E-Z 96® Plant DNA Kit. DNA was extracted from 50 mg plant leaf tissue from the following sources: lane 1 from sweet potato, lane 2 from tomato, lane 3 from maize, lane 4 from peanut, lane 5 from corn, lane 6 from rice, lane 7 from strawberry and lane 8 from pea. Cellular DNA (10% of total purified DNA) was analyzed on a 0.8% agarose gel to demonstrate yield and purity. M: Lambda Hind III DNA Marker.