Mag-Bind® FFPE DNA/RNA 96 Kit
High-Throughput DNA & RNA Extraction from FFPE Tissue
Mag-Bind® FFPE DNA/RNA 96 Kit is designed for the sequential isolation of DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes non-toxic mineral oil in the place of the hazardous xylene for efficient deparaffinization of the FFPE sample. The specially formulated buffers reverse cross-linking without the need for overnight digestion resulting in high-yielding, high-quality nucleic acids. The isolation protocol allows for the extraction of both DNA and RNA in separate eluates from the same sample for a comprehensive analysis of both the nucleic acids. Purified DNA and RNA are suitable for a variety of downstream applications including SNP analysis, sequencing, and genotyping. The Mag-Bind® system is fully automatable on the Hamilton Microlab® STAR™, Tecan Fluent™, Thermo KingFisher® Flex™, and other open-ended workstations.
- Xylene-free protocol
- Extraction of both DNA and RNA in separate eluates from the same FFPE sample
- No splitting of samples
- Magnetic bead-based purification
- Automation friendly
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Applications||NGS, PCR, qPCR, real-time RT-PCR, microarray, microRNA analysis|
|Elution Volume||50-200 µL|
|Starting Material||FFPE tissue|
|Starting Amount||< 3 FFPE sections of 10 μm thickness each|
|Throughput||Up to 96|
|Processing Mode||Automated, Manual|
Protocol and Resources
Product Documentation & Literature
Figure 1. Average DNA (A) and RNA (B) yields from FFPE tumor samples. Genomic DNA (A) and RNA (B) was sequentially isolated from the same 1 × 10 μm section of the FFPE tumor tissue sample (n=3) using Omega Bio-tek’s Mag-Bind® FFPE DNA/RNA 96 Kit and comparable kits from Company T and Company Q following manufacturer’s recommended protocols. Purified DNA and RNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system as well as Promega’s QuantiFluor® dsDNA and RNA system.
Figure 2. Purity of DNA (A) and RNA (B) isolated using different manufacturer’s kits was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.
Figure 3. Purity of DNA (A) and RNA (B) isolated using different manufacturer’s kits was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.
Table 1. Average DV200 value (percentage of fragments >200 nt) of RNA purified using different kits analyzed on Agilent’s TapeStation® 2200.
Table 2. Average DV200 value (percentage of fragments >200 nt) of RNA purified using different kits analyzed on Agilent’s TapeStation® 2200.
Table 3. ΔCq values of DNA extracted from FFPE and non-FFPE colorectal tumor tissue samples using kits from different manufacturers.
Figure 4. Small Variant Calling Analysis on DNA from FFPE colorectal tumor tissue sample extracted from different kits compared to fresh frozen control.