Category Archives: Application Notes

Saliva as a Diagnostic Tool for COVID-19: A Proof-of-Concept Study

Application Note Kiranmai Durvasula1, Havva Keskin1, Julie Baggs1, Travis Butts1 1Omega Bio-tek, Inc, Norcross GA 30071 Introduction To combat a fast-evolving global pandemic like COVID-19, it is essential to develop rapid and accurate diagnostic tests with a quick turnaround time. In recent times, saliva has emerged as a viable alternate sample source to nasopharyngeal swabs […]

Automated Circulating Cell-Free DNA Extraction from 8 mL Sample Volumes

Application Note Kiranmai Durvasula1, Jeff Roeder1, Chris Lee1, Travis Butts1 1Omega Bio-tek, Inc. Norcross, GA 30071 Introduction Circulating cell-free DNA (cfDNA) holds great clinical significance for non-invasive disease detection, diagnosis and monitoring. cfDNA are usually small fragments of DNA (size distribution peaking at ~170 bp) found circulating in plasma, serum, or other bodily fluids and […]

A Complete, Walk Away DNA Purification Solution from 10 mL Whole Blood using Omega Bio-tek’s Reagents on the Hamilton Microlab® STAR™

Application Note Kiranmai Durvasula1, Jeff Roeder1, Chris Lee1, Travis Butts1 1Omega Bio-tek, Inc. Norcross, GA 30071 Introduction With advances in genomics research, personalized medicine and sequencing-based technologies, there is a necessity for purification of high-quality genomic DNA from large volumes of blood. The rapidly growing landscape of biorepositories that store large amounts of DNA from […]

High Throughput Solution for DNA Extraction from Stool Samples Using Magnetic Beads

Application Note Kiranmai Durvasula1, Travis Butts1 1Omega Bio-tek, Inc. Introduction Stool samples are often preferred clinical specimens for detection of multiple disease-causing microbes, biomarker detection of cancers of the digestive tract to analysis of gut microbiome and their impact on physiological health and disease. The sample collection is relatively easy, non-invasive, and amenable for large sample sizes. Stool, however, is […]

Genomic DNA Normalization Using Omega Bio-tek’s Mag-BIND® EquiPure gDNA Normalization Kit

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1 1Omega Bio-tek, Inc. Introduction DNA normalization is a crucial step for any next-generation sequencing or PCR-based analysis workflows. Quantification and normalization of DNA is traditionally done either by UB absorbance or fluorescent nucleic acid stain-based methods requiring tedious processes of quantification, computation, and concentration adjustment. Omega Bio-tek’s […]

Comprehensive, High Throughput Workflow for Automated gDNA Isolation from iSWAB Oral Samples

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1, Bassam El-Fahmawi2 1Omega Bio-tek, Inc., 2MAWI DNA Technologies Introduction High quality DNA is a critical factor in determining success of many molecular biology applications, such as next-generation sequencing (NGS), qPCR, microarrays, etc. Other potential applications include clinical molecular diagnostics, genetic analyses, pharmacogenomics, animal speciation studies, and forensics. […]

A Performance Comparsion Study: Omega Bio-tek’s E.Z.N.A.® HP Total RNA Kit (R6812) vs. Qiagen’s RNeasy Plus Mini Kit (74134)

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1 1Omega Bio-tek, Inc. Introduction Extraction of sufficient amounts of high quality RNA is a crucial step in generating the most sensitive and relevant results downstream. Among a variety of commercially available extraction kits on the market, we pit the performance of our E.Z.N.A.® HP Total RNA Kit […]

Superior Performance of Omega Bio-tek’s E.Z.N.A.® Soil DNA Kit Over Company M’s Soil DNA Isolation Kit for DNA Extraction from Soil Samples

Application Note Introduction Molecular analysis of soil DNA offers a direct solution for detecting microorganisms residing in soil and studying microbial diversity. Isolation of DNA from soils is often challenging because of the presence of many contaminants like humic acid that can interfere with the extraction process and are inhibitory to several downstream applications. An […]

Complete High Throughput Pharmacogenomics Workflow: From Sample to Results

Application Note Michael Farrell1,Jeff Roeder1, Travis Butts1, Julie Baggs1, Luke Linz2, Mike Freeman2, Steven Nesbit3, Joan Gordon3 1Omega Bio-tek, Inc., 2Douglas Scientific., 3Maine Molecular Quality Controls, Inc. Introduction Rapid, high throughput, cost-effective and accurate screening of human DNA for the identification of single nucleotide polymorphisms (SNPs) is a useful tool for the development of therapeutics […]

Reliable Isolation of DNA from Complex Food Matrices Using Omega Bio-tek’s E.Z.N.A.® Food DNA Kit

Application Note Kiranmai Durvasula1, Julie Baggs1, Travis Butts1 1Omega Bio-tek, Inc., Norcross GA 30071 Introduction Food DNA testing is essential to assess quality, safety, and authenticity of the food for regulatory compliance, accurate labeling, and public interest. The first crucial step in the food DNA testing workflow is the extraction of high quality DNA. Omega […]