Monthly Archives: January 2018

Genomic DNA Normalization Using Omega Bio-tek’s Mag-BIND® EquiPure gDNA Normalization Kit

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1 1Omega Bio-tek, Inc. Introduction DNA normalization is a crucial step for any next-generation sequencing or PCR-based analysis workflows. Quantification and normalization of DNA is traditionally done either by UB absorbance or fluorescent nucleic acid stain-based methods requiring tedious processes of quantification, computation, and concentration adjustment. Omega Bio-tek’s […]

Comprehensive, High Throughput Workflow for Automated gDNA Isolation from iSWAB Oral Samples

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1, Bassam El-Fahmawi2 1Omega Bio-tek, Inc., 2MAWI DNA Technologies Introduction High quality DNA is a critical factor in determining success of many molecular biology applications, such as next-generation sequencing (NGS), qPCR, microarrays, etc. Other potential applications include clinical molecular diagnostics, genetic analyses, pharmacogenomics, animal speciation studies, and forensics. […]

A Performance Comparsion Study: Omega Bio-tek’s E.Z.N.A.® HP Total RNA Kit (R6812) vs. Qiagen’s RNeasy Plus Mini Kit (74134)

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1 1Omega Bio-tek, Inc. Introduction Extraction of sufficient amounts of high quality RNA is a crucial step in generating the most sensitive and relevant results downstream. Among a variety of commercially available extraction kits on the market, we pit the performance of our E.Z.N.A.® HP Total RNA Kit […]

Superior Performance of Omega Bio-tek’s E.Z.N.A.® Soil DNA Kit Over Company M’s Soil DNA Isolation Kit for DNA Extraction from Soil Samples

Application Note Introduction Molecular analysis of soil DNA offers a direct solution for detecting microorganisms residing in soil and studying microbial diversity. Isolation of DNA from soils is often challenging because of the presence of many contaminants like humic acid that can interfere with the extraction process and are inhibitory to several downstream applications. An […]

Complete High Throughput Pharmacogenomics Workflow: From Sample to Results

Application Note Michael Farrell1,Jeff Roeder1, Travis Butts1, Julie Baggs1, Luke Linz2, Mike Freeman2, Steven Nesbit3, Joan Gordon3 1Omega Bio-tek, Inc., 2Douglas Scientific., 3Maine Molecular Quality Controls, Inc. Introduction Rapid, high throughput, cost-effective and accurate screening of human DNA for the identification of single nucleotide polymorphisms (SNPs) is a useful tool for the development of therapeutics […]

Reliable Isolation of DNA from Complex Food Matrices Using Omega Bio-tek’s E.Z.N.A.® Food DNA Kit

Application Note Kiranmai Durvasula1, Julie Baggs1, Travis Butts1 1Omega Bio-tek, Inc., Norcross GA 30071 Introduction Food DNA testing is essential to assess quality, safety, and authenticity of the food for regulatory compliance, accurate labeling, and public interest. The first crucial step in the food DNA testing workflow is the extraction of high quality DNA. Omega […]

High Throughput Workflows for DNA Extractions from Oral Samples

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1, Mike Farrell1 1Omega Bio-tek, Inc., Norcross GA 30071 Introduction With advances in genomics research and clinical diagnostics, there is a need for rapid, high throughput and cost-effective technologies for purifying high quality DNA from cheek samples. DNA Genotek’s ORAcollect (Figure 1) offers a painless, non-invasive method for […]

A Simple, User-Friendly, High Throughput DNA Extraction Workflow Using 2-D Barcoded Buccal Swabs on Hamilton’s Firefly NIMBUS® 96

Application Note Kiranmai Durvasula1, Jeff Roeder1, Travis Butts1 1Omega Bio-tek, Inc., Norcross GA 30071 Introduction Buccal swabs are one of the most commonly used tools for collecting DNA samples because of their non-invasiveness, ease of collection and transportation. Compared to other sampling alternatives such as blood, buccal sampling is cost-effective, pain-free and can be easily […]